Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Palmitoylcarnitine"

From Bioblast
(11 intermediate revisions by 5 users not shown)
Line 1: Line 1:
{{MitoPedia
{{MitoPedia
|abbr=Pal
|abbr=Pal
|description='''Palmitoyl carnitine''' is an ester derivative of [[carnitine]] (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production.
|description='''Palmitoylcarnitine''' is an ester derivative of [[carnitine]] (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production.
|type=Substrate ETS
|type=Substrate ETS
}}
__TOC__
'''Carnitine palmitoyltransferase I''' (CPT1) also known as '''carnitine acyltransferase I''' or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine.
== Application in [[HRR]] ==
{{Chemical_description
|abbr=Pal
|trivial name=Palmitoylcarnitine
|complete name=Palmitoyl-DL-carnitine-HCl
|chem formula=C<sub>23</sub>H<sub>46</sub>NO<sub>4</sub> · Cl
|molar mass=436.07
|vendor=Sigma-Aldrich
|product number=P4509
|store at=-20 °C
|sensitivity=
|cas=6865-14-1
|h statements=
|h info=
}}<!--:::'''Pal: Palmitoylcarnitine'''  (Palmitoyl-DL-carnitine-HCl, C<sub>23</sub>H<sub>45</sub>N0<sub>4</sub>HCl); Sigma P 4509, 100 mg, store at -20 °C; M = 436.1 g·mol<sup>-1</sup>.-->
::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
::::# Weigh 8.72 mg of palmitoylcarnitine.
::::# Dissolve in 2 mL H<sub>2</sub>O.
::::# Divide into 0.2 mL portions.
::::# Store frozen at -20 °C.
:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
::::* Titration volume: 8 µL using a 25 µL syringe (2 mL O2k-chamber).
::::* Final concentration: 0.04 mM.
{{MitoPedia methods|type=Substrate ETS
}}
}}
{{MitoPedia topics
{{MitoPedia topics
Line 8: Line 42:
|type=Substrate ETS
|type=Substrate ETS
}}
}}
 
{{Labeling
== Application in [[HRR]]  ==
|additional=MitoPedia:FAT4BRAIN
 
}}
'''Pal: Palmitoyl carnitine'''  (Palmitoyl-DL-carnitine-HCl, C<sub>23</sub>H<sub>45</sub>N0<sub>4</sub>HCl); Sigma P 4509,  100 mg, store at -20 °C; FW = 436.1
 
'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
 
::1) weigh 8.72 mg of palmitoyl carnitine
::2) dissolve in 2 ml H<sub>2</sub>O
::3) divide into 0.2 ml portions
::4) store frozen at -20 °C

Revision as of 16:07, 18 January 2022


high-resolution terminology - matching measurements at high-resolution


Palmitoylcarnitine

Description

Palmitoylcarnitine is an ester derivative of carnitine (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production.

Abbreviation: Pal

Carnitine palmitoyltransferase I (CPT1) also known as carnitine acyltransferase I or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine.


Application in HRR

Pal: Palmitoylcarnitine (Palmitoyl-DL-carnitine-HCl; C23H46NO4 · Cl), Sigma-Aldrich: P4509, store at -20 °C, CAS: 6865-14-1, M = 436.07 g·mol-1


Preparation of 10 mM stock solution (dissolved in H2O):
  1. Weigh 8.72 mg of palmitoylcarnitine.
  2. Dissolve in 2 mL H2O.
  3. Divide into 0.2 mL portions.
  4. Store frozen at -20 °C.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume: 8 µL using a 25 µL syringe (2 mL O2k-chamber).
  • Final concentration: 0.04 mM.



MitoPedia topics: Substrate and metabolite 


Labels:






MitoPedia:FAT4BRAIN