Difference between revisions of "Pecinova 2011 Mitochondrion"
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{{Publication | {{Publication | ||
|title=Pecinova A, Drahota Z, Nuskova H, Pecina P, Houstek J (2011) Evaluation of basic mitochondrial functions using rat tissue homogenates. Mitochondrion 11: 722- | |title=Pecinova A, Drahota Z, Nuskova H, Pecina P, Houstek J (2011) Evaluation of basic mitochondrial functions using rat tissue homogenates. Mitochondrion 11:722-8. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/21664301 PMID:21664301] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/21664301 PMID:21664301] [https://www.researchgate.net/publication/51210702_Evaluation_of_basic_mitochondrial_functions_using_rat_tissue_homogenates Open Access] | ||
|authors=Pecinova A, Drahota Z, Nuskova H, Pecina P, Houstek J | |authors=Pecinova A, Drahota Z, Nuskova H, Pecina P, Houstek J | ||
|year=2011 | |year=2011 | ||
|journal=Mitochondrion | |journal=Mitochondrion | ||
|abstract=The primary attempt in diagnostic and experimental studies of numerous pathological states associated with mitochondrial dysfunction is a precise evaluation of changes in function, content and structure of mitochondrial OXPHOS system. The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential, and BN/SDS-PAGE western blotting demonstrated that tissue homogenates can substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50-100mg of the tissue is required for the complex OXPHOS analysis, i.e. five times less as compared with isolated mitochondria. | |abstract=The primary attempt in diagnostic and experimental studies of numerous pathological states associated with mitochondrial dysfunction is a precise evaluation of changes in function, content and structure of mitochondrial OXPHOS system. The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential, and BN/SDS-PAGE western blotting demonstrated that tissue homogenates can substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50-100mg of the tissue is required for the complex OXPHOS analysis, i.e. five times less as compared with isolated mitochondria. | ||
|keywords=Isolated mitochondria | |keywords=Isolated mitochondria, Tissue homogenates, oxidative phosphorylation, Respiratory control, Mitochondrial membrane potential | ||
|mipnetlab=CZ Prague Houstek J | |mipnetlab=CZ Prague Houstek J, CZ Hradec Kralove Cervinkova Z | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |||
|tissues=Heart, Nervous system, Liver, Kidney | |||
|preparations=Homogenate, Isolated mitochondria | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=N, ROX | |||
|instruments=Oxygraph-2k, TPP | |instruments=Oxygraph-2k, TPP | ||
}} | }} | ||
Latest revision as of 15:35, 13 November 2017
| Pecinova A, Drahota Z, Nuskova H, Pecina P, Houstek J (2011) Evaluation of basic mitochondrial functions using rat tissue homogenates. Mitochondrion 11:722-8. |
Pecinova A, Drahota Z, Nuskova H, Pecina P, Houstek J (2011) Mitochondrion
Abstract: The primary attempt in diagnostic and experimental studies of numerous pathological states associated with mitochondrial dysfunction is a precise evaluation of changes in function, content and structure of mitochondrial OXPHOS system. The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential, and BN/SDS-PAGE western blotting demonstrated that tissue homogenates can substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50-100mg of the tissue is required for the complex OXPHOS analysis, i.e. five times less as compared with isolated mitochondria. • Keywords: Isolated mitochondria, Tissue homogenates, oxidative phosphorylation, Respiratory control, Mitochondrial membrane potential
• O2k-Network Lab: CZ Prague Houstek J, CZ Hradec Kralove Cervinkova Z
Labels:
Organism: Rat
Tissue;cell: Heart, Nervous system, Liver, Kidney
Preparation: Homogenate, Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: N, ROX
HRR: Oxygraph-2k, TPP
