Pesta 2010 Abstract IOC60
| 1. Desplanches D, Hoppeler H, Tüscher L, Mayet MH, Spielvogel H, Ferretti G, et al. (1996) Muscle tissue adaptations of high-altitude natives to training in chronic hypoxia or acute normoxia. J Appl Physiol 81:1946-51.
2. Hoppeler H, Klossner S, Vogt M (2008) Training in hypoxia and its effects on skeletal muscle tissue. Scand J Med Sci Sports 18 Suppl 1:38-49. 3. Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle: new perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837-45. 4. Schocke MF, Esterhammer R, Arnold W, Kammerlander C, Burtscher M, Fraedrich G, et al. (2005) High-energy phosphate metabolism during two bouts of progressive calf exercise in humans measured by phosphorus-31 magnetic resonance spectroscopy. Eur J Appl Physiol 93:469-79. |
» IOC60
Pesta D, Jacobs R, Macek C, Hoppel F, Faulhaber M, Lundby C, Burtscher M, Gnaiger E, Schocke M (2010) Mitochondr. Physiol. Network
Abstract: Skeletal muscle is a highly adaptable tissue that can adjust to different stimuli [1,2]. In the present study we investigated the impact of endurance training on muscle oxidative capacity with high resolution respirometry [3] and 31P magnetic resonance spectroscopy (31P MRS)[4]. 40 healthy untrained subjects (UG) who performed an endurance training program 3 times a week lasting for 10 weeks were included in the study. 17 highly trained athletes (AG) were studied for comparison. Spatially-resolved dynamic 31P MRS measurements were obtained from the upper leg and biopsy samples were taken from the vastus lateralis to assess mitochondrial capacity with high-resolution respirometry. Subsequently, endurance and strength capacities of the subjects were determined via motor performance tests. After 10 weeks, the initial tests and muscle biopsies were repeated. We observed a significant increase in mass specific OXPHOS flux with training in the UG from 78.97 ± 16.05 to 101.39 ± 19.19 pmol.s-1.mg-1 (p<0.01). Flux between the UG and AG, both before and after training, respectively, was significantly different (AG: 120 ± 32.79 pmol.s-1.mg-1, UG see above, p<0.01). The capacity of the mitochondria to oxidize MCFA was significantly increased with training, observed as an increase in absolute flux (from 12.79 ± 4.67 pre-training to 29.58 ± 7.25 pmol.s-1.mg-1 post-training, p<0.01) and in the flux control ratio (FCR=fraction of a given flux relative to the maximal flux) of octanoyl-carnitine (0.14 ± 0.05 pre-training to 0.28 ± 0.04 post-training, p<0.01). However, no difference in the FCR of MCFA oxidation was found between UG after training and the AG (0.28 ± 0.04 post-training vs 0.26 ± 0.06). The FCR of OXPHOS was increased after training (0.95 ± 0.09, p<0.01) but was not different between UG before training and AG (0.86 ± 0.09 pre-training vs. 0.86 ± 0.11). To date, analysis of the 31P MRS was still in progress. In conclusion, mitochondria seem to adapt to endurance training in a quantitative and qualitative way. The qualitative adaptations can most prominently be observed in the capacity of MCFA oxidation, which is increased due to training. The limitation of the OXPHOS system seems to be decreased temporarily in untrained subjects exposed to exercise training. Yet, the exact reason for this reversed decreased limitation in athletic subjects is unknown. • Keywords: Exercise Training, Respiration, Permeabilized Fibres, Skeletal Muscle
Labels:
Stress:Hypoxia Organism: Human Tissue;cell: Skeletal Muscle"Skeletal Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property. Preparation: Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Enzyme Enzyme: Complex I, Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property., Complex III, Complex IV; Cytochrome c Oxidase"Complex IV; Cytochrome c Oxidase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property., Complex V; ATP Synthase"Complex V; ATP Synthase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Flux Control; Additivity; Threshold; Excess Capacity"Flux Control; Additivity; Threshold; Excess Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Fatty Acid"Fatty Acid" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
Event: IOC60"IOC60" is not in the list (A1, A2, A3, A4, B1, B2, B3, B4, C1, C2, ...) of allowed values for the "Event" property.
