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Pesta 2023 MiP2023

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MiPschool 2023

Pesta 2023 MiP2023

Dominik Pesta
Respiratory capacity of skeletal muscle and peripheral blood mononuclear cells of male and female individuals with type 2 diabetes.

Link: MiP2023 Obergurgl AT

Pesta Dominik (2023)

Event: MiP2023 Obergurgl AT

Authors: Buescher F-M, Schrage-Knoll Irmtrud, Bohmeier Maria, Kaiser-Stolz C, Kramme J, Rittweger J, Pesta Dominik
Introduction: Skeletal muscle mitochondrial function is altered in insulin resistant states. Its assessment, however, requires invasive muscle biopsies to obtain viable tissue for functional mitochondrial analysis. Blood cell-based bioenergetics potentially reflects systemic mitochondrial function. Here, we characterized respiratory capacity of skeletal muscle mitochondria and peripheral blood mononuclear cells (PBMCs) from patients with type 2 diabetes and assessed whether the latter reflect muscle mitochondrial respirometric measures.
Methods: For that purpose, 20 patients with type 2 diabetes (30 % female, 57±9 years, BMI 28±4 kg/m2) participated in this study. We obtained muscle biopsies from the M. vastus lateralis and venous blood samples to isolate PBMCs. High-resolution respirometry was performed in duplicate to assess mitochondrial respiration from permeabilized muscle fibers and PBMCs using an established SUIT-protocol.
Results and Discussion: Combined NADH-linked (N) electron transfer and succinate-linked (S) OXPHOS capacity was 59.4±13.0 pmol/(s*mg) for muscle and 16.6±5.3 pmol/(s*106 cells) for PBMCs. NS-OXPHOS capacity was not different between females and males for muscle (66.5±9.5 vs 56.3±13.0 pmol/(s*mg), p=0.10) or PBMCs (19.5±5.3 vs 15.3±5.0 pmol/(s*106), p=0.10), respectively. While PBMC mitochondrial function was not correlated with skeletal muscle respiratory function across several respiratory states (all p>0.05), muscle NS-OXPHOS capacity correlated negatively with diabetes disease duration (r=-0.50, p=0.02). These results suggest that there are no sex-specific differences with regard to muscle and PBMC mitochondrial function in individuals with type 2 diabetes. While bioenergetic phenotypes in PBMCs do not reflect muscle mitochondrial function in this cohort, diabetes disease duration negatively associates with muscle mitochondrial function.

Keywords: mitochondrial function, type 2 diabetes, blood cells, skeletal muscle

O2k-Network Lab: DE Cologne Pesta D

Affiliations and acknowledgements

Büscher F-M1, Schrage I1, Bohmeier M1, Kaiser-Stolz C1, Kramme J1,2, Rittweger J1, Pesta D1,2,3
  1. German Aerospace Center (DLR), Institute of Aerospace Medicine, D-51147 Cologne, Germany.
  2. Center for Endocrinology, Diabetes and Preventive Medicine (CEDP), University Hospital Cologne, D-50937 Cologne, Germany.
  3. Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, D-50931 Cologne, Germany.
Corresponding author: [email protected]
We wish to thank all patients and staff that participated in or helped with this study.

Labels: Pathology: Diabetes 

Tissue;cell: Skeletal muscle, Blood cells 

HRR: Oxygraph-2k  Event: Oral