Difference between revisions of "Podrabsky 2000 Am J Physiol Regul Integr Comp Physiol"
| Line 3: | Line 3: | ||
|authors=Podrabsky JE, Javillonar C, Hand SC, Crawford DL | |authors=Podrabsky JE, Javillonar C, Hand SC, Crawford DL | ||
|year=2000 | |year=2000 | ||
|journal= Am. J. Physiol. | |journal=Am. J. Physiol. | ||
|keywords= | |abstract=A previous phylogenetic | ||
analysis among 15 taxa of the teleost fish Fundulus suggested | |||
that there should be thermal-adaptive differences in | |||
heart metabolism among populations. To test this hypothesis, | |||
the rate of oxygen consumption and the activities of all 11 | |||
glycolytic enzymes were measured in isolated heart ventricle | |||
from two populations of Fundulus heteroclitus. Heart ventricular | |||
metabolism is greater in a northern population versus a | |||
southern population of these fish. Analysis of the amount of | |||
glycolytic enzymes indicates that 87% of the variation in | |||
cardiac metabolism within and between populations is explained | |||
by the variation in three enzymes (pyruvate kinase, | |||
glyceraldehyde-3-phosphate dehydrogenase, and lactate dehydrogenase). | |||
These enzymes are the same three enzymes | |||
that were predicted to be important based on previously | |||
determined phylogenetic patterns of expression. Our data | |||
indicate that near-equilibrium enzymes, as well as classically | |||
defined rate-limiting enzymes, can also influence metabolism. | |||
|keywords=Evolution, Cardiac adaptation | |||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/11080103 PMID: 11080103] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/11080103 PMID: 11080103] | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Fish | |||
|tissues=Cardiac Muscle | |||
|topics=Respiration; OXPHOS; ETS Capacity | |topics=Respiration; OXPHOS; ETS Capacity | ||
|instruments=CaloRespirometry; Twin-Flow, DatLab Software; Separate Application, Method | |instruments=CaloRespirometry; Twin-Flow, DatLab Software; Separate Application, Method | ||
}} | }} | ||
Revision as of 13:50, 19 October 2010
| Podrabsky JE, Javillonar C, Hand SC, Crawford DL (2000) Intraspecific variation in aerobic metabolism and glycolytic enzyme expression in heart ventricles. Am. J. Physiol. 279: R2344-R2348. |
Podrabsky JE, Javillonar C, Hand SC, Crawford DL (2000) Am. J. Physiol.
Abstract: A previous phylogenetic analysis among 15 taxa of the teleost fish Fundulus suggested that there should be thermal-adaptive differences in heart metabolism among populations. To test this hypothesis, the rate of oxygen consumption and the activities of all 11 glycolytic enzymes were measured in isolated heart ventricle from two populations of Fundulus heteroclitus. Heart ventricular metabolism is greater in a northern population versus a southern population of these fish. Analysis of the amount of glycolytic enzymes indicates that 87% of the variation in cardiac metabolism within and between populations is explained by the variation in three enzymes (pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase, and lactate dehydrogenase). These enzymes are the same three enzymes that were predicted to be important based on previously determined phylogenetic patterns of expression. Our data indicate that near-equilibrium enzymes, as well as classically defined rate-limiting enzymes, can also influence metabolism. • Keywords: Evolution, Cardiac adaptation
Labels:
Organism: Fish"Fish" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Tissue;cell: Cardiac Muscle"Cardiac Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: CaloRespirometry; Twin-Flow"CaloRespirometry; Twin-Flow" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., DatLab Software; Separate Application"DatLab Software; Separate Application" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Method"Method" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
