Difference between revisions of "Porter 2022 Abstract Bioblast"
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{{Abstract | {{Abstract | ||
|title=Karavyraki M, Gnaiger E, Porter | |title=[[File:Richie.jpg|left|100px|Porter Richard K]] Karavyraki M, Gnaiger E, <u>Porter Richard K</u> (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. Bioblast 2022: BEC Inaugural Conference. | ||
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | |info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | ||
|authors=Karavyraki Marilena, Gnaiger Erich, Porter Richard K | |authors=Karavyraki Marilena, Gnaiger Erich, Porter Richard K | ||
|year=2022 | |year=2022 | ||
|event=Bioblast 2022 | |event=Bioblast 2022 | ||
|abstract=In an endeavour to understand the metabolic phenotype behind metastasis from oral squamous cell carcinomas, we characterised the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial | |abstract=In an endeavour to understand the metabolic phenotype behind metastasis from oral squamous cell carcinomas, we characterised the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial density but lower mitochondrial O<sub>2</sub> flow per cell ''J''<sub>O<sub>2</sub></sub> than DOK cells. The lower cell ''J''<sub>O<sub>2</sub></sub> in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial density. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux. | ||
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|keywords=Oral Squamous Cancer Cells, Mitochondria, Interleukin 6, Dysplastic oral keratinocytes, Oxygen consumption | |keywords=Oral Squamous Cancer Cells, Mitochondria, Interleukin 6, Dysplastic oral keratinocytes, Oxygen consumption | ||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
}} | }} | ||
== Affiliations and support == | == Affiliations and support == | ||
::::Marilena Karavyraki | :::: Marilena Karavyraki<sup>1</sup>, Erich Gnaiger<sup>2</sup>, Richard K Porter<sup>1</sup> | ||
::::#School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland. | ::::# School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - rkporter@tcd.ie | ||
::::#Oroboros Instruments, Innsbruck, Austria | ::::# Oroboros Instruments, Innsbruck, Austria | ||
::::Acknowledgements: Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 (2016-2021) | ::::Acknowledgements: Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 [[MitoEAGLE]] (2016-2021). | ||
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Revision as of 08:37, 27 May 2022
Karavyraki M, Gnaiger E, Porter Richard K (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. Bioblast 2022: BEC Inaugural Conference. |
Link: Bioblast 2022: BEC Inaugural Conference
Karavyraki Marilena, Gnaiger Erich, Porter Richard K (2022)
Event: Bioblast 2022
In an endeavour to understand the metabolic phenotype behind metastasis from oral squamous cell carcinomas, we characterised the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial density but lower mitochondrial O2 flow per cell JO2 than DOK cells. The lower cell JO2 in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial density. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux.
β’ Keywords: Oral Squamous Cancer Cells, Mitochondria, Interleukin 6, Dysplastic oral keratinocytes, Oxygen consumption β’ Bioblast editor: Plangger M
Affiliations and support
- Marilena Karavyraki1, Erich Gnaiger2, Richard K Porter1
- School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - [email protected]
- Oroboros Instruments, Innsbruck, Austria
- Marilena Karavyraki1, Erich Gnaiger2, Richard K Porter1
- Acknowledgements: Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 MitoEAGLE (2016-2021).
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