Rausser 2021 Elife: Difference between revisions
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== Correction: beta-Oxidation and CII ambiguity == | == Correction: beta-Oxidation and CII ambiguity == | ||
:::: beta-Oxidation is shown to be linked to CI (correct) and CII (wrong) in a Figure published in refs. [1,2,3,4]. For clarification, see ref. [ | :::: beta-Oxidation is shown to be linked to CI (correct) and CII (wrong) in a Figure published in refs. [1,2,3,4]. For clarification, see ref. [5]. | ||
:::# Picard M, Prather AA, Puterman E, Cuillerier A, Coccia M, Aschbacher K, Burelle Y, Epel ES (2018) A mitochondrial health index sensitive to mood and caregiving stress. '''Biol Psychiatry''' 84:9-17. - [[Picard 2018 Biol Psychiatry |ยปBioblast linkยซ]] | :::# Picard M, Prather AA, Puterman E, Cuillerier A, Coccia M, Aschbacher K, Burelle Y, Epel ES (2018) A mitochondrial health index sensitive to mood and caregiving stress. '''Biol Psychiatry''' 84:9-17. - [[Picard 2018 Biol Psychiatry |ยปBioblast linkยซ]] |
Revision as of 23:39, 12 September 2023
Rausser S, Trumpff C, McGill MA, Junker A, Wang W, Ho SH, Mitchell A, Karan KR, Monk C, Segerstrom SC, Reed RG, Picard M (2021) Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures. Elife 10:e70899. https://doi.org/10.7554/eLife.70899 |
Rausser S, Trumpff C, McGill MA, Junker A, Wang W, Ho SH, Mitchell A, Karan KR, Monk C, Segerstrom SC, Reed RG, Picard M (2021) Elife
Abstract: Using a high-throughput mitochondrial phenotyping platform to quantify multiple mitochondrial features among molecularly defined immune cell subtypes, we quantify the natural variation in mitochondrial DNA copy number (mtDNAcn), citrate synthase, and respiratory chain enzymatic activities in human neutrophils, monocytes, B cells, and naรฏve and memory T lymphocyte subtypes. In mixed peripheral blood mononuclear cells (PBMCs) from the same individuals, we show to what extent mitochondrial measures are confounded by both cell type distributions and contaminating platelets. Cell subtype-specific measures among women and men spanning four decades of life indicate potential age- and sex-related differences, including an age-related elevation in mtDNAcn, which are masked or blunted in mixed PBMCs. Finally, a proof-of-concept, repeated-measures study in a single individual validates cell type differences and also reveals week-to-week changes in mitochondrial activities. Larger studies are required to validate and mechanistically extend these findings. These mitochondrial phenotyping data build upon established immunometabolic differences among leukocyte subpopulations, and provide foundational quantitative knowledge to develop interpretable blood-based assays of mitochondrial health.
โข Bioblast editor: Gnaiger E
Correction: beta-Oxidation and CII ambiguity
- beta-Oxidation is shown to be linked to CI (correct) and CII (wrong) in a Figure published in refs. [1,2,3,4]. For clarification, see ref. [5].
- Picard M, Prather AA, Puterman E, Cuillerier A, Coccia M, Aschbacher K, Burelle Y, Epel ES (2018) A mitochondrial health index sensitive to mood and caregiving stress. Biol Psychiatry 84:9-17. - ยปBioblast linkยซ
- Karan KR, Trumpff C, McGill MA, Thomas JE, Sturm G, Lauriola V, Sloan RP, Rohleder N, Kaufman BA, Marsland AL, Picard M (2020) Mitochondrial respiratory capacity modulates LPS-induced inflammatory signatures in human blood. Brain Behav Immun Health 5:100080. - ยปBioblast linkยซ
- Bindra S, McGill MA, Triplett MK, Tyagi A, Thaker PH, Dahmoush L, Goodheart MJ, Ogden RT, Owusu-Ansah E, R Karan K, Cole S, Sood AK, Lutgendorf SK, Picard M (2021) Mitochondria in epithelial ovarian carcinoma exhibit abnormal phenotypes and blunted associations with biobehavioral factors. Sci Rep 11:11595. - ยปBioblast linkยซ
- Rausser S, Trumpff C, McGill MA, Junker A, Wang W, Ho SH, Mitchell A, Karan KR, Monk C, Segerstrom SC, Reed RG, Picard M (2021) Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures. Elife 10:e70899. - ยปBioblast linkยซ
- Gnaiger E (2023) Complex II ambiguities โ FADH2 in the electron transfer system. MitoFit Preprints 2023.3.v6. https://doi.org/10.26124/mitofit:2023-0003.v6
Labels: MiParea: mtDNA;mt-genetics
Organism: Human
Tissue;cell: Blood cells, Platelet
Enzyme: Complex II;succinate dehydrogenase, Marker enzyme
Pathway: F
PBMCs