Difference between revisions of "Resorufin"
From Bioblast
(Created page with "{{MitoPedia |description='''Resorufin''' is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the [[Horseradish_peroxidase| Hors...") ย |
|||
Line 1: | Line 1: | ||
'''Resorufin''' is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the [[Horseradish_peroxidase| Horseradish peroxidase-catalyzed assay using [[Amplex Red]] for the measurement of H<sub>2</sub>O<sub>2</sub> production. | |||
ย | |||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Fluorometry | |mitopedia method=Fluorometry |
Revision as of 09:47, 18 April 2014
Resorufin is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the [[Horseradish_peroxidase| Horseradish peroxidase-catalyzed assay using Amplex Red for the measurement of H2O2 production.
MitoPedia methods:
Fluorometry
Preparation of Resorufin Solutions
Source: Sigma 424455
Storage solution
Prepare a 1.2 mM storage solution of resorufin by adding 13.5 mg resorufin in 50 ml of a 10 mM KOH solution and store aliquots of 1 ml or 2 ml of this storage solution at -20 ยฐC.
Stock solution
Prior to experiments thaw 2 ml storage solution, pipette 1,666 ยตl into a 25 ml volumetric flask and add 10 mM KOH to 50 ml to obtain a stock solution of 80 ยตM resorufin.