Difference between revisions of "Reverse electron flow from CII to CI"
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* <div>[ | * <div>[https://wiki.oroboros.at/index.php/Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways]</div> | ||
* [https://wiki.oroboros.at/index.php/Komlodi_2018_J_Bioenerg_Biomembr Komlodi_2018_J_Bioenerg_Biomembr] | * [https://wiki.oroboros.at/index.php/Komlodi_2018_J_Bioenerg_Biomembr Komlodi_2018_J_Bioenerg_Biomembr] | ||
* [https://wiki.oroboros.at/index.php/Makrecka-Kuka_2015_Biomolecules Makrecka-Kuka_2015_Biomolecules] | * [https://wiki.oroboros.at/index.php/Makrecka-Kuka_2015_Biomolecules Makrecka-Kuka_2015_Biomolecules] | ||
Revision as of 14:16, 3 August 2020
- high-resolution terminology - matching measurements at high-resolution
Reverse electron flow from CII to CI
Description
Reverse electron flow from CII to CI stimulates production of ROS when mitochondria are incubated with succinate without rotenone in the LEAK state. Depolarisation of mitochondrial membrane potential (e.g. after ADP addition to stimulate OXPHOS) leads to inhibition of RET and therefore, decrease of RET-initiated ROS production. RET can be also measured when mitochondria are respiring on Gp without rotenone in the LEAK state. Addition of IQ-side inhibitors (ubiquinone-binding side of CI) of CI usually block RET. The following SUIT protocols allows you to measure RET-initiated H2O2 flux in mitochondrial preparations: SUIT-009 and SUIT-026.
Abbreviation: RET
Reference:
MitoPedia topics: Enzyme
