Rhodamine 123

high-resolution terminology - matching measurements at high-resolution

Rhodamine 123

Description

Rhodamine 123 is an extrinsic fluorophore and can be used as a probe to determine changes in mitochondrial membrane potential. Rh123 is a lipophilic cation that is accumulated by mitochondria in proportion to Δψ_mt. Using ethanol as the solvent, the excitation maximum is511 nm and the emission maximum is 534 nm.The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively (Sigma-Aldrich).

Abbreviation: Rh123

Application in HRR

Life Technologies, R302, 25 mg, store at -20 °C, FW=380.83

Preparation of 5 mM storage solution (dissolved in ethanol)

1. Weight 1.9 mg of Rh123 and dissolve in 1 mL ethanol;
2. Divide into 0.1 mL portions (use dark vials);
3. Store at -20 °C.

Preparation of 0.2 mM stock solution

1. Dilute a 100 µL aliquot 5 mM storage solution with 2400 µL ethanol to reach 2.5 mL final volume;
2. Divide into 0.2 mL portions (use dark vials);
3. Store at -20 °C.

Signal and output

1. Signal: The O2k-Fluo LED2-Module is operated through the Amperometric (Amp)-Channel of the O2k, with electric current (ampere [A]) as the primary signal.
2. Output: The focus of the output with Rh123 is on Type C: Force, mt-membrane potential.

Rh123 with the O2k-FluoRespirometer: exploratory experiments

MitoPedia methods: Fluorometry 

Labels: MiParea: Respiration, Instruments;methods 

HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol 

O2k-MultiSensor