Difference between revisions of "S/NS pathway control ratio"
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=C<sub>II</sub>/C<sub>I+II</sub> | ||
|description=The ''' | |description=The '''C<sub>II</sub>/C<sub>I+II</sub> [[substrate control ratio]]''' is obtained when [[rotenone]] (Rot) is added to [[CI+II |C<sub>I+II</sub>]]-linked respiration in a defined coupling state. The reversed protocol, adding C<sub>I</sub>-linked substrates to a C<sub>II</sub>-linked [[background state]] does not provide a valid estimation of C<sub>II</sub>-linked respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (C<sub>II</sub>). | ||
|info=[[Gnaiger 2009 Int J Biochem Cell Biol]], [[Gnaiger 2012 MitoPathways]] | |info=[[Gnaiger 2009 Int J Biochem Cell Biol]], [[Gnaiger 2012 MitoPathways]] | ||
}} | }} | ||
Revision as of 10:04, 25 June 2014
- high-resolution terminology - matching measurements at high-resolution
S/NS pathway control ratio
Description
The CII/CI+II substrate control ratio is obtained when rotenone (Rot) is added to CI+II-linked respiration in a defined coupling state. The reversed protocol, adding CI-linked substrates to a CII-linked background state does not provide a valid estimation of CII-linked respiration with succinate in the absence of Rot, since oxaloacetate accumulates as a potent inhibitor of succinate dehydrogenase (CII).
Abbreviation: CII/CI+II
Reference: Gnaiger 2009 Int J Biochem Cell Biol, Gnaiger 2012 MitoPathways
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
