From Bioblast
Description
SF 6847 (C18H22N2O), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent uncoupler of oxidative phosphorylation, being used in the nM range. Like all uncouplers, SF 6847 concentrations must be titrated carefully to evaluated the optimum concentration for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations.
Abbreviation: SF6847
Reference: Chinopoulos 2014 Methods Enzymol, Terada 1984 Biochim Biophys Acta
MitoPedia topics:
Uncoupler
Application in HRR
Preparation
- SF 6847/tyrphostin A9/malonoben (C18H22N2O, [[3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenyl]methylene]propanedinitrile; Sigma T182, 5 mg: FW = 282.38. Alternative source: Enzo, BML-EI215.
- Caution: Toxic!
- Preparation of 50 mM stock solution (dissolved in 99.9% ethanol):
- To 5 mg in a glass vial: dissolve with 354.1 Β΅L ethanol.
- Store in glass vials at -20 Β°C.
- Preparation of 500 Β΅M stock solution (dissolved in 99.9% ethanol):
- Add 990 Β΅L ethanol and 10 Β΅L of the 50 mM stock solution to a glass vial.
- Store in glass vials at -20 Β°C.
- Preparation of 50 Β΅M stock solution (dissolved in 99.9% ethanol):