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Revision as of 10:05, 26 January 2024 by Cardoso Luiza (talk | contribs) (Created page with "{{MitoPedia |description=300 px |info='''A''': protocol for determination of O<sub><small>2</small></sub> flux in mitochondrial preparation...")
- high-resolution terminology - matching measurements at high-resolution
SUIT-006 02 mt D108
Description
Reference: A: protocol for determination of O2 flux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- SUIT-006
SUIT number: D108_mt;1PGM;2D;2c;3U;4Myx
O2k-Application: O2
in preparation Communicated by Cardoso Luiza HD, Grings M (last update 2024-01-26)
Representative traces
Strengths and limitations
- Control protocol of SUIT-006 NADH mt D084 for respiration only, allowing for cytochrome c test.
- + Reasonable duration of the experiment.
- - Careful washing is required after the experiment to avoid carry-over of inhibitors. The addition of sample homogenate is recommended in the washing protocol to bind strong inhibitors.
- After myxothyazol titration, this protocol can be extended with the Complex IV assay.
Compare SUIT protocols
- SUIT-006 NADH mt D084: Similar protocol for simultaneous determination of O2 flux and NADH autofluorescence in isolated mitochondria.
Chemicals and syringes
Template:Chemical SUIT-006 O2 mt D108
- Suggested stock concentrations are shown in the specific DL-Protocol.
