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Difference between revisions of "SUIT protocol pattern"

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|mitopedia O2k and high-resolution respirometry=SUIT protocol
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Β  Author: [[Gnaiger Erich]] 2016-01-31
Author: [[Gnaiger Erich]] 2016-01-31
== Linear ==
== Linear ==



Revision as of 16:34, 13 May 2016


high-resolution terminology - matching measurements at high-resolution


SUIT protocol pattern

Description

The SUIT protocol pattern describes the type of the sequence of coupling and substrate control steps in a SUIT protocol, which may be liner, orthogonal, or diametral.

Abbreviation: SUITp-Pattern

Reference: MiPNet21.06 SUIT reference protocol


MitoPedia concepts: MiP concept, SUIT concept, "MitoFit Quality Control System" is not in the list (MiP concept, Respiratory state, Respiratory control ratio, SUIT concept, SUIT protocol, SUIT A, SUIT B, SUIT C, SUIT state, Recommended, ...) of allowed values for the "MitoPedia concept" property. MitoFit Quality Control System"MitoFit Quality Control System" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 






Author: Gnaiger Erich 2016-01-31

Linear

  1. Linear coupling control: at a single substrate state (classical State 3 -> State 4, or P -> LT).
  2. Linear substrate control: at a single coupling state (unusual).
  3. Monodirectional linear coupling control: LN -> PD -> EF
  4. Bidirectional linear coupling control: LN -> PD -> LOmy -> EF


Orthogonal

  1. Orthogonal coupling -> substrate control (mono- or bidirectional)
  2. Orthogonal substrate -> coupling control (mono- or bidirectional)


Diametral

  1. Diametral coupling -> substrate ->> control (mono- or bidirectional)
  2. Diametral substrate -> coupling ->> control (mono- or bidirectional)