SUIT reference protocol: Difference between revisions
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The cross-linked respiratory states for harmonization between protocols are: | The cross-linked respiratory states for harmonization between protocols are: | ||
* [[SUIT-RP1]]:9Gp, [[SUIT-RP2]]:10Rot - [[SGp]]<sub>''E''</sub> | * [[SUIT-RP1]]:9Gp, [[SUIT-RP2]]:10Rot - [[SGp]]<sub>''E''</sub> | ||
* 11Tm and 12Tm - [[Complex IV single step | | * 11Tm and 12Tm - [[Complex IV single step |Tm]]<sub>''E''</sub> | ||
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[[File:SUIT-Reference-Protocol.jpg|700px |SUIT RP]]<br /> | [[File:SUIT-Reference-Protocol.jpg|700px |SUIT RP]]<br /> | ||
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Revision as of 14:28, 11 February 2016
Description
The substrate-uncoupler-inhibitor titration (SUIT) reference protocol, SUIT-RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT-RP consists of two harmonized SUIT protocols (SUIT-RP1 and SUIT-RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.
Abbreviation: SUIT-RP
Reference: MiPNet21.06 SUIT reference protocol
MitoPedia O2k and high-resolution respirometry: "SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property.
SUIT protocol"SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property.
Cross-linked respiratory states
The cross-linked respiratory states for harmonization between protocols are: