Difference between revisions of "Sartor 2004 Gen Physiol Biophys"
| Line 10: | Line 10: | ||
cell situations. | cell situations. | ||
|keywords=Rat lactotrope cells, Cell energetics, Aerobic and anaerobic pathways, pHi , [Cl−]i | |keywords=Rat lactotrope cells, Cell energetics, Aerobic and anaerobic pathways, pHi , [Cl−]i | ||
|mipnetlab=FR_Pessac_Beauvoit B, FR Bordeaux Rigoulet M, | |mipnetlab=FR_Pessac_Beauvoit B, FR Bordeaux Rigoulet M, FR Bordeaux Devin A | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
Revision as of 15:43, 25 February 2013
| Sartor P, Madec F, Garcia L, Dejean L, Beauvoit B, Dufy B, Rigoulet M (2004) Interactions between intracellular chloride concentrations, intracellular pH and energetic status in rat lactotrope cells in primary culture. Gen Physiol Biophys 23: 195-208. |
Sartor P, Madec F, Garcia L, Dejean L, Beauvoit B, Dufy B, Rigoulet M (2004) Gen Physiol Biophys
Abstract: Rat lactotrope cells in primary cultures have a higher intracellular Cl− concentration ([Cl−]i) than that predicted by a passive distribution across the membrane. This suggests that active cellular mechanisms ensure this ionic equilibrium. In this study, we examined the interactions between pHi, [Cl−]i regulation and cell energetics. We analyzed: 1. the interactions between extracellular Cl−concentrations, [Cl−]i and cellular energy; 2. the influence of [Cl−]i on respiratory chain function; 3. the correlation with glycolysis and; 4. the role played by pHi in these cellular mechanisms. We show that low [Cl−]i decreases ATP cell content, ATP/ADP ratio and modify phosphorylative oxidations. ATP production is rather due to the anaerobic pathway of the glucose metabolism than the aerobic one and depends also on other metabolic substrates among which glutamine probably has a special role. Finally, pHi appears as a determinant in the balance between aerobic and anaerobic pathways. These results are discussed in relation to the role of Cl− in normal and pathological (effect of hypoxia on mature and immature neurons) cell situations. • Keywords: Rat lactotrope cells, Cell energetics, Aerobic and anaerobic pathways, pHi, [Cl−]i
• O2k-Network Lab: FR_Pessac_Beauvoit B, FR Bordeaux Rigoulet M, FR Bordeaux Devin A
Labels:
Stress:Hypoxia Organism: Rat Tissue;cell: Neurons; Brain"Neurons; Brain" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property. Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
