Difference between revisions of "Siebels 2013 Biochim Biophys Acta"
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{{Publication | {{Publication | ||
|title=Siebels I, Dröse S (2013) Q-site inhibitor induced ROS production of mitochondrial complex II is attenuated by TCA cycle dicarboxylates. Biochim Biophys Acta 1827:1156-1164. | |title=Siebels I, Dröse S (2013) Q-site inhibitor induced ROS production of mitochondrial complex II is attenuated by TCA cycle dicarboxylates. Biochim Biophys Acta 1827:1156-1164. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/23800966 PMID: 23800966] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/23800966 PMID: 23800966] | ||
|authors=Siebels I, Dröse S | |authors=Siebels I, Dröse S | ||
|year=2013 | |year=2013 | ||
|journal=Biochim Biophys Acta | |journal=Biochim Biophys Acta | ||
|abstract=The impact of complex II (succinate:ubiquinone oxidoreductase) on the mitochondrial production of reactive oxygen species (ROS) has been underestimated for a long time. However, recent studies with intact mitochondria revealed that complex II can be a significant source of ROS. Using submitochondrial particles from bovine heart mitochondria as a system that allows the precise setting of substrate concentrations we could show that mammalian complex II produces ROS at subsaturating succinate concentrations in the presence of Q-site inhibitors like atpenin A5 or when a further downstream block of the respiratory chain occurred. Upon inhibition of the ubiquinone reductase activity, complex II produced about 75% hydrogen peroxide and 25% superoxide. ROS generation was attenuated by all dicarboxylates that are known to bind competitively to the substrate binding site of complex II, suggesting that the oxygen radicals are mainly generated by the unoccupied flavin site. Importantly, the ROS production induced by the Q-site inhibitor atpenin A5 was largely unaffected by the redox state of the Q pool and the activity of other respiratory chain complexes. Hence, complex II has to be considered as an independent source of mitochondrial ROS in physiology and pathophysiology. | |abstract=The impact of complex II (succinate:ubiquinone oxidoreductase) on the mitochondrial production of reactive oxygen species (ROS) has been underestimated for a long time. However, recent studies with intact mitochondria revealed that complex II can be a significant source of ROS. Using submitochondrial particles from bovine heart mitochondria as a system that allows the precise setting of substrate concentrations we could show that mammalian complex II produces ROS at subsaturating succinate concentrations in the presence of Q-site inhibitors like atpenin A5 or when a further downstream block of the respiratory chain occurred. Upon inhibition of the ubiquinone reductase activity, complex II produced about 75% hydrogen peroxide and 25% superoxide. ROS generation was attenuated by all dicarboxylates that are known to bind competitively to the substrate binding site of complex II, suggesting that the oxygen radicals are mainly generated by the unoccupied flavin site. Importantly, the ROS production induced by the Q-site inhibitor atpenin A5 was largely unaffected by the redox state of the Q pool and the activity of other respiratory chain complexes. Hence, complex II has to be considered as an independent source of mitochondrial ROS in physiology and pathophysiology. | ||
|keywords=2-n-decyl-quinazolin-4-yl-amine, 2-thenoyltrifluoroacetone, Atpenin A5, Complex II, DQA, Dicarboxylates, FAD, HRP, Mitochondria, NAD(+)/NADH, Q, Q(o) site, Q-site, QFR, RET, ROS, Reactive oxygen species (ROS), SMP, SOD, SQR, Succinate:ubiquinone oxidoreductase, TTFA, flavin adenine dinucleotide, horseradish peroxidase, oxidized/reduced from of nicotinamide adenine dinucleotide, quinol:fumarate oxidoreductases, reactive oxygen species, reverse electron transfer, submitochondrial particles, succinate:quinone oxidoreductases, superoxide dismutase, ubiquinol oxidation site of cytochrome bc(1) complex (complex III), ubiquinone, ubiquinone reduction site of complex II | |keywords=2-n-decyl-quinazolin-4-yl-amine, 2-thenoyltrifluoroacetone, Atpenin A5, Complex II, DQA, Dicarboxylates, FAD, HRP, Mitochondria, NAD(+)/NADH, Q, Q(o) site, Q-site, QFR, RET, ROS, Reactive oxygen species (ROS), SMP, SOD, SQR, Succinate:ubiquinone oxidoreductase, TTFA, flavin adenine dinucleotide, horseradish peroxidase, oxidized/reduced from of nicotinamide adenine dinucleotide, quinol:fumarate oxidoreductases, reactive oxygen species, reverse electron transfer, submitochondrial particles, succinate:quinone oxidoreductases, superoxide dismutase, ubiquinol oxidation site of cytochrome bc(1) complex (complex III), ubiquinone, ubiquinone reduction site of complex II | ||
|mipnetlab=DE Frankfurt Brandt U | |mipnetlab=DE Frankfurt Brandt U | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Medicine | |||
|organism=Bovines | |||
|tissues=Heart | |||
|injuries=RONS; Oxidative Stress | |||
|substratestates=CI, CII | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Revision as of 12:07, 3 February 2014
| Siebels I, Dröse S (2013) Q-site inhibitor induced ROS production of mitochondrial complex II is attenuated by TCA cycle dicarboxylates. Biochim Biophys Acta 1827:1156-1164. |
Siebels I, Dröse S (2013) Biochim Biophys Acta
Abstract: The impact of complex II (succinate:ubiquinone oxidoreductase) on the mitochondrial production of reactive oxygen species (ROS) has been underestimated for a long time. However, recent studies with intact mitochondria revealed that complex II can be a significant source of ROS. Using submitochondrial particles from bovine heart mitochondria as a system that allows the precise setting of substrate concentrations we could show that mammalian complex II produces ROS at subsaturating succinate concentrations in the presence of Q-site inhibitors like atpenin A5 or when a further downstream block of the respiratory chain occurred. Upon inhibition of the ubiquinone reductase activity, complex II produced about 75% hydrogen peroxide and 25% superoxide. ROS generation was attenuated by all dicarboxylates that are known to bind competitively to the substrate binding site of complex II, suggesting that the oxygen radicals are mainly generated by the unoccupied flavin site. Importantly, the ROS production induced by the Q-site inhibitor atpenin A5 was largely unaffected by the redox state of the Q pool and the activity of other respiratory chain complexes. Hence, complex II has to be considered as an independent source of mitochondrial ROS in physiology and pathophysiology. • Keywords: 2-n-decyl-quinazolin-4-yl-amine, 2-thenoyltrifluoroacetone, Atpenin A5, Complex II, DQA, Dicarboxylates, FAD, HRP, Mitochondria, NAD(+)/NADH, Q, Q(o) site, Q-site, QFR, RET, ROS, Reactive oxygen species (ROS), SMP, SOD, SQR, Succinate:ubiquinone oxidoreductase, TTFA, flavin adenine dinucleotide, horseradish peroxidase, oxidized/reduced from of nicotinamide adenine dinucleotide, quinol:fumarate oxidoreductases, reactive oxygen species, reverse electron transfer, submitochondrial particles, succinate:quinone oxidoreductases, superoxide dismutase, ubiquinol oxidation site of cytochrome bc(1) complex (complex III), ubiquinone, ubiquinone reduction site of complex II
• O2k-Network Lab: DE Frankfurt Brandt U
Labels: MiParea: Respiration, mt-Medicine
Stress:RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Bovines Tissue;cell: Heart
HRR: Oxygraph-2k
