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Property "Has-20abstract" (as page type) with input value "-27-27'Kuznetsov-20AV,-20Gnaiger-20E-20(2010)-20Oxygraph-20assay-20of-20cytochrome-20-27-27c-27-27-20oxidase-20activity:-20chemical-20background-20correction.-20Mitochondr-20Physiol-20Network-2006.06(07):1-2D4.-27-27'-20-0A-0AAutoxidation-20of-20reduced-20compounds-20(such-20as-20ascorbate,-20TMPD,-20cytochrome-20c)-20causes-20a-20chemical-20background-20oxygen-20flux,-20which-20is-20a-20function-20of-20oxygen-20concentration-20and-20has-20to-20be-20subtracted-20fom-20total-20oxygen-20flux.-20The-20new-20DatLab-20software-20provides-20on-2Dline-20correction-20for-20instrumental-20and-20chemical-20background.-0A-0AOxygraphic-20determination-20of-20cytochrome-20c-20oxidase-20activity-20in-20the-20presence-20of-20TMPD,-20ascorbate-20and-20cytochrome-20c-20requires-20consideration-20of-20chemical-20background-20oxygen-20consumption.-20Several-20compounds-20are-20readily-20oxidized-20by-20molecular-20oxygen-20when-20dissolved-20in-20water.-20This-20leads-20to-20a-20significant-20chemical-20oxygen-20consumption-20in-20the-20absence-20of-20any-20respiring-20biological-20sample.-20The-20rate-20of-20this-20autoxidation-20represents-20a-20chemical-20background-20which-20strongly-20depends-20on-20experimental-20conditions,-20such-20as-20temperature,-20chemical-20composition,-20pH-20and-20oxygen-20concentration.-20Moreover,-20the-20rate-20of-20autoxidation-20may-20be-20significantly-20catalyzed-20by-20metal-20traces-20and-20metal-2Dcontaining-20proteins-20(e.g.-20cytochrome-20c).-20Therefore,-20high-2Dresolution-20respirometric-20measurement-20of-20oxygen-20flux-20ultimately-20depends-20on-20chemical-20background-20correction-20analyzed-20under-20experimental-20conditions-20over-20the-20entire-20oxygen-20range.-0A-0AAfter-20sequential-20titration-20of-20ascorbate-20(added-20first)-20and-20TMPD-20(with-20or-20without-20added-20cytochrome-20c),-20the-20total-20oxygen-20flux-20increases-20due-20to-20(i)-20COX-20activity-20and-20(ii)-20autooxidation-20of-20ascrobate-20and-20TMPD.-20Correction-20for-20autooxidation-20is-20a-20routine-20procedure-20in-20high-2Dresolution-20respirometry,-20but-20it-20requires-20precautions.-20We-20recommend,-20therefore,-20evaluation-20of-20chemical-20background-20effects-20by-20application-20of-20inhibitors-20of-20cytochrome-20c-20oxidase-20(cyanide,-20azide)-20subsequent-20to-20recording-20of-20flux-20with-20ascorbate-20and-20TMPD.-20After-20full-20inhibitioin-20of-20COX,-20a-20small-20non-2DCOX-20component-20of-20biological-20oxidations-20and-20the-20large-20effect-20of-20autooxidation-20are-20recorded-20over-20a-20range-20of-20further-20declining-20oxygen-20concentration.-20After-20re-2Doxygenation,-20inhibited-20oxygen-20flux-20is-20recorded-20at-20oxygen-20levels-20above-20and-20within-20the-20oxygen-20concentration-20pertaining-20to-20the-20COX-20activity-20measurment.-20This-20provides-20a-20complete-20internal-20calibration-20of-20the-20chemical-20background,-20as-20a-20function-20of-20oxygen-20concentration.-0A:-3E-3E-20Product:-20O2k-2DCatalogue:-20-5B-5BO2k-2DMultiSensor-5D-5D,-20-5B-5BO2k-2DCore-5D-5D,-20-5B-5BO2k-2DCatalogue-5D-5D" contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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