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Property "Has-20abstract" (as page type) with input value "Mitochondrial-20membrane-20potential-20(Δψm)-20plays-20important-20roles-20in-20the-20normal-20function-20of-20cells-20and-20in-20pathobiochemical-20situations.-20The-20application-20of-20ion-2Dselective-20electrodes-20for-20the-20measurement-20of-20Δψm-20-20is-20important-20for-20studying-20normal-20biological-20reactions-20and-20pathways-20and-20mitochondrial-20diseases.-20We-20constructed-20and-20optimized-20a-20computerized-20device-20for-20real-2Dtime-20monitoring-20of-20the-20Δψm,-20which-20included-20modification-20of-20tetraphenylphosphonium-20(TPP+)-2Dselective-20membrane-20that-20improved-20reproducibility-20of-20the-20TPP+-2Dselective-20electrode.-20Application-20of-20MATLAB-20software-20increased-20the-20sensitivity-20of-20the-20system.-20We-20tested-20our-20improved-20device-20for-20membrane-20potential-20measurements-20of-20isolated-20mitochondria-20(in-20absolute-20scale-20of-20millivolts).-20In-20addition,-20we-20assessed-20relative-20changes-20of-20Δψm-20(as-20changes-20in-20TPP+-20-20concentration)-20of-20digitonin-2Dpermeabilized-20cells-20(hepatocytes,-20control-20transmitochondrial-20cybrids,-20HeLa-20G-20and-20BSC-2D40)-20after-20addition-20of-20substrates,-20inhibitors,-20and-20uncoupler-20of-20respiratory-20chain.-20Our-20system-20can-20be-20successfully-20used-20for-20studies-20of-20many-20aspects-20of-20the-20regulation-20of-20mitochondrial-20bioenergetics-20and-20as-20a-20diagnostic-20tool-20for-20mitochondrial-20oxidative-20phosphorylation-20disorders." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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