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Property "Has-20abstract" (as page type) with input value "Mitochondrial-20respiratory-20function-20was-20studied-20in-20permeabilized-20pig-20liver-20biopsies.-20The-20cell-20membrane-20was-20permeabilized-20mechanically-20in-20tissue-20samples-20of-202–7-20mg,-20for-20application-20of-20a-20standardized-20substrate-2Finhibitor-20titration-20protocol-20in-20high-2Dresolution-20respirometry.-20Specific-20respirometric-20tests-20demonstrated-20complete-20plasma-20membrane-20permeabilization-20and-20accessibility-20of-20substrates-20to-20intact-20mitochondria.-20High-20respiratory-20adenylate-20control-20ratios-20and-20cytochrome-20c-20conservation-20in-20the-20tissue-20preparation-20were-20comparable-20or-20even-20better-20than-20in-20isolated-20mitochondria.-20Citrate-20synthase-20and-20cytochrome-20c-20oxidase-20activities-20remained-20at-2085-25-20of-20controls-20after-20up-20to-2098-20h-20storage-20of-20liver-20tissue-20at-200°C-20in-20histidine–tryptophan–ketoglutarate-20solution.-20Multiple-20mitochondrial-20defects,-20however,-20were-20indicated-20after-2048-20h-20cold-20storage-20by-20the-20decline-20in-20respiratory-20capacity,-20which-20was-20lowered-20to-20a-20larger-20extent-20with-20complex-20I-20substrates-20compared-20to-20respiration-20with-20substrates-20for-20complex-20II-20or-20IV,-20measured-20in-20the-20absence-20of-20cytochrome-20c.-20After-20prolonged-20ischemia,-20the-20adenylate-20control-20ratio-20was-20significantly-20reduced,-20and-20cytochrome-20c-20depletion-20was-20detected-20by-20the-20stimulatory-20effect-20of-20cytochrome-20c.-20High-2Dresolution-20respirometry-20allows-20the-20assessment-20of-20mitochondrial-20function-20in-20a-20few-20milligrams-20of-20permeabilized-20liver-20tissue,-20without-20isolation-20of-20mitochondria.-20This-20provides-20a-20basis-20for-20the-20analysis-20of-20mitochondrial-20function-20in-20human-20liver-20biopsies." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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