Search by property

Property "Has-20abstract" (as page type) with input value "Triphenylphosphonium-2Dconjugated-20imidazole-2Dsubstituted-20oleic-20acid-20(TPP-2DIOA)-20is-20an-20inhibitor-20of-20cytochrome-20c-20peroxidase-20activity-20that-20appears-20to-20initiate-20apoptotic-20cell-20death-20via-20cytochrome-20-27-27c-27-27-20release.-20As-20a-20molecule-20that-20interacts-20directly-20with-20cytochrome-20-27-27c-27-27,-20TPP-2DIOA-20may-20also-20affect-20mitochondrial-20respiration.-20We-20are-20evaluating-20the-20dose-2Deffect-20relationships-20of-20TPP-2DIOA’s-20interactions-20with-20mitochondria.-0A-0AAlthough-20original-20data-20came-20from-20cells-20maintained-20in-20glucose-20media-20in-20culture,-20the-20interpretation-20of-20these-20data-20is-20limited-20by-20the-20fact-20that-20under-20these-20conditions-20the-20cells-20derive-20little-20ATP-20from-20oxidative-20phosphorylation-20and-20have-20low-20mitochondrial-20densities.-20-20We-20are-20revisiting-20the-20effects-20of-20TPP-2DIOA-20on-20mitochondrial-20respiration-20and-20apoptotic-20cell-20death-20in-20glucose-2Dfree-20media,-20in-20which-20energy-20is-20derived-20from-20galactose-20and-20glutamine-20and-20cells-20are-20highly-20reliant-20on-20oxidative-20phosphorylation.-20-20Cells-20growing-20under-20these-20conditions-20are-20highly-20sensitive-20to-20drugs-20that-20may-20perturb-20oxidative-20phosphorylation.-20We-20are-20also-20evaluating-20the-20effects-20of-203-2Dhydroxypropyl-2DTPP-20(TPP),-20the-20TPP-20moiety-20of-20TPP-2DIOA,-20on-20mitochondrial-20function-20in-20this-20system-20and-20in-20isolated-20mitochondria.-0AAt-20concentrations-20that-20effectively-20inhibit-20mitochondrial-2Dmediated-20cell-20death-20in-20cells,-20TPP-2DIOA-20exerted-20significant-20effects-20on-20oxidative-20phosphorylation,-20e.g.-20reducing-20the-20respiratory-20control-20ratio.-20Conjugation-20to-20TPP-20is-20frequently-20used-20to-20promote-20accumulation-20of-20molecules-20in-20mitochondria.-20However,-20since-20TPP-20and-20linker-20molecules-20can-20themselves-20have-20effects-20on-20mitochondrial-20function-20it-20is-20necessary-20to-20evaluate-20these-20independently,-20an-20experimental-20control-20that-20is-20often-20not-20performed.-20We-20find-20similar-20effects-20of-20TPP-20and-20TPP-2DIOA-20on-20mitochondrial-20OXPHOS,-20suggesting-20that-20it-20is-20the-20TPP-20moiety-20that-20interferes-20with-20normal-20mitochondrial-20function-20in-20this-20assay.-0A-0ATaken-20together,-20these-20preliminary-20data-20indicate-20the-20importance-20of-20(i)-20choosing-20appropriate-20cell-20culture-20conditions-20that-20promote-20reliance-20on-20oxidative-20phosphorylation-20to-20evaluate-20the-20effects-20of-20molecules-20that-20are-20targeted-20to-20mitochondria,-20and-20(ii)-20including-20experimental-20controls-20of-20TPP,-20since-20this-20moiety-20and-20linker-20group-20can-20have-20significant-20effects-20on-20mitochondrial-20function." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

Showing below up to 0 results starting with #1.

View (previous 50 | next 50) (20 | 50 | 100 | 250 | 500)