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Property "Has-20abstract" (as page type) with input value "When-20long-2Dchain-20fatty-20acids-20enter-20the-20cells,-20long-2Dchain-20acyl-2DCoA-20synthases-20(ACSLs)-20convert-20them-20to-20acyl-2DCoAs-20in-20an-20ATP-2Ddependent-20reaction.-20The-20resulting-20acyl-2DCoAs-20have-20numerous-20metabolic-20routes-20within-20cells,-20including-20incorporation-20into-20triacylglycerol-20(TAG)-20and-20membrane-20phospholipids.-20Acyl-2DCoAs-20are-20used-20as-20substrates-20for-20beta-2Doxidation-20and-20protein-20acylation-20and-20function-20as-20ligands-20for-20transcription-20factors.-20However,-20the-20function-20of-20ACSL6-20in-20skeletal-20muscle-20cells-20has-20not-20been-20described.-20The-20aim-20of-20this-20study-20was-20to-20investigate-20the-20effects-20of-20ACSL6-20knockdown-20on-20mitochondrial-20metabolism-20in-20skeletal-20muscle-20cells.-0A Isolation-20of-20primary-20rat-20skeletal-20muscle-20cells-20from-20the-20lower-20limb-20was-20performed-20by-20collagenase-20II-20digestion-20-5B1-5D.-20Knockdown-20of-20ACSL6-20was-20made-20by-20siRNA-20specific-20transfection.-20After-20the-20knockdown,-20the-20cells-20were-20collected-20for-20the-20following-20experiments:-20mRNA-20expression-20(RT-2DPCR),-20MS-2DMS-20lipid-20analyzes,-20cell-20viability-20(flow-20cytometry),-20oxygen-20consumption-20(Oroboros-20Oxygraph-2D2k)-20-5B2-5D-20and-20reactive-20oxygen-20species-20(ROS)-20production-20(Amplex-20UltraRed).-0A ACSL6-20siRNA-20transfection-20(20-20nM)-20reduced-20the-20expression-20of-20ACSL6-20mRNA-20by-2070±8-25.-20ACSL6-20knockdown-20increased-20the-20free-20fatty-20acids-20C16:0-20and-20C18:0-20by-2032±3-25-20and-2035±3-25,-20respectively.-20siRNA-20transfection-20did-20not-20affect-20cell-20viability-20measured-20by-20propide-20iodate.-20ACSL6-20genic-20silencing-20increased-20mitochondrial-20respiration-20in-20all-20states-20-5Bpmol-20O2∙s-3Csup-3E−1-3C-2Fsup-3E∙10-3Csup-3E−6-3C-2Fsup-3E-20cells-5D:-20ROUTINE-20respiration-20(297±30-20vs-20368±28),-20LEAK-20with-20oligomycin-20(91±5-20vs-2096±4)-20and-20noncoupled-20ET-2Dpathway-20(610±45-20vs-20703±41),-20and-20decreased-20ROS-20production-20(-27-27P-27-27-3C0.05).-20ACSL6-20genic-20silencing-20increased-20mRNA-20expression-20of-20oxidative-20genes-20PGC1-20(~50-25),-20UCP2-20(~3-20fold)-20and-20UCP3-20(~5-20fold),-20decreased-20mRNA-20expression-20of-20ACSL3-20and-20had-20no-20effect-20on-20ACSL1-20and-20β-2Dhydroxyacyl-2DCoA-20dehydrogenase-20(β-2DHAD).-20-0A ACSL6-20knockdown-20increased-20the-20availability-20of-20free-20fatty-20acids,-20which-20are-20major-20regulators-20of-20UCP’s.-20This-20may-20reflect-20the-20action-20of-20signaling-20pathways-20which-20remodel-20the-20oxidative-20program-20of-20skeletal-20muscle-20cells,-20increasing-20mitochondrial-20respiration.-20These-20mechanisms-20may-20contribute-20to-20control-20metabolic-20diseases,-20such-20as-20insulin-20resistance-20and-20obesity." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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