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Difference between revisions of "State 2"

From Bioblast
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|description=Substrate limited state of [[residual oxygen consumption]], after addition of ADP (ROX<sub>D</sub>).Β  Residual endogenous substrates are oxidized during a transient stimulation of oxygen flux by ADP; ADP concentration (D) remains high during ROX<sub>D</sub>.
|description=Substrate limited state of [[residual oxygen consumption]], after addition of ADP (ROX<sub>D</sub>).Β  Residual endogenous substrates are oxidized during a transient stimulation of oxygen flux by ADP; ADP concentration (D) remains high during ROX<sub>D</sub>.


In contrast to the definition of State 2 by Chance and Williams (1955, 1956), a respiratory [[LEAK]] state may be induced as the second respiratory state in a protocol with isolated mitochondria, permeabilized tissues, or permeabilized cells, adding the mitochondrial preparation to respiration medium containing inorganic phosphate (State 1), then adding reduced substrate (no external adenylates, State 2'; whereas State 2 is defined as high [ADP] without external substrates). This second state is a non-phosphorylating LEAK state, ''L''<sub>N</sub> (N for no adenylates), when substrate-saturated respiration compensates for the proton leak (mainly) in the absence of ADP.
In contrast to the definition of '''State 2''' by Chance and Williams (1955, 1956), a respiratory [[LEAK]] state may be induced as the second respiratory state in a protocol with isolated mitochondria, permeabilized tissues, or permeabilized cells, adding the mitochondrial preparation to respiration medium containing inorganic phosphate (State 1), then adding reduced substrate (no external adenylates, State 2'; whereas State 2 is defined as high [ADP] without external substrates). This second state is a non-phosphorylating LEAK state, ''L''<sub>N</sub> (N for no adenylates), when substrate-saturated respiration compensates for the proton leak (mainly) in the absence of ADP.
|info=[[MiPNet12.15]], Chance 1955 JBC-III
|info=[[MiPNet12.15]], Chance 1955 JBC-III
|type=Respiration
|type=Respiration

Revision as of 01:11, 31 January 2011


high-resolution terminology - matching measurements at high-resolution


State 2

Description

Substrate limited state of residual oxygen consumption, after addition of ADP (ROXD). Residual endogenous substrates are oxidized during a transient stimulation of oxygen flux by ADP; ADP concentration (D) remains high during ROXD.

In contrast to the definition of State 2 by Chance and Williams (1955, 1956), a respiratory LEAK state may be induced as the second respiratory state in a protocol with isolated mitochondria, permeabilized tissues, or permeabilized cells, adding the mitochondrial preparation to respiration medium containing inorganic phosphate (State 1), then adding reduced substrate (no external adenylates, State 2'; whereas State 2 is defined as high [ADP] without external substrates). This second state is a non-phosphorylating LEAK state, LN (N for no adenylates), when substrate-saturated respiration compensates for the proton leak (mainly) in the absence of ADP.

Abbreviation: ROXD

Reference: MiPNet12.15, Chance 1955 JBC-III


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Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.