Difference between revisions of "State 2"
| Line 10: | Line 10: | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|kinetics=ADP; Pi, Reduced Substrate; Cytochrome c | |kinetics=ADP; Pi, Reduced Substrate; Cytochrome c | ||
|topics=Respiration; OXPHOS; ETS Capacity | |topics=Respiratory state, Respiration; OXPHOS; ETS Capacity | ||
|type=Respiration | |type=Respiration | ||
}} | }} | ||
Revision as of 19:21, 24 February 2011
- high-resolution terminology - matching measurements at high-resolution
State 2
Description
Substrate limited state of residual oxygen consumption, after addition of ADP to isolated mitochondria suspended in mitochondrial respiration medium in the absence of reduced substrates (ROXD). Residual endogenous substrates are oxidized during a transient stimulation of oxygen flux by ADP; ADP concentration (D) remains high during ROXD.
In contrast to the definition of State 2 by Chance and Williams (1955, 1956), a respiratory LEAK state may be induced as the second respiratory state in a protocol with isolated mitochondria, permeabilized tissues, or permeabilized cells, adding the mitochondrial preparation to respiration medium containing inorganic phosphate (State 1), then adding reduced substrate (no external adenylates, State 2'; whereas State 2 is defined as high [ADP] without external substrates). This second state is a non-phosphorylating LEAK state, LN (N for no adenylates), when substrate-saturated respiration compensates for the proton leak (mainly) in the absence of ADP.
Abbreviation: ROXD
Reference: MiPNet12.15, Chance 1955 JBC-III
Labels:
Regulation: Respiratory state"Respiratory state" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
