- high-resolution terminology - matching measurements at high-resolution
State 3
Description
State 3
Abbreviation: P
Reference: Chance_1955_JBC-III; MiPNet12.15
MitoPedia methods:
Respirometry,
Spectrophotometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Maximum oxygen flux in State 3 ?
Oxygen flux is not necessarily 'maximum' in State 3 for different reasons:
- Concentrations of ADP and inorganic phosphate (Pi) are high but may not be saturating (kinetic limitation).
- The substrate or substrate combination may not support the physiological maximum flux through the electron transfer system (ETS), particularly due to electron gating of mitochondrial pathways converging at the Q-junction (limitation due to substrate control).
- OXPHOS capacity is less than ETS capacity in coupled mitochondria with limiting phosphorylation system capacity (limitation by the enzymatic capacity of utilizating the electrochemical proton gradient).
