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Difference between revisions of "Steady state"

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{{MitoPedia
{{MitoPedia
|description=A system is in a steady state if the levels of a specific variable (e.g. O<sub>2</sub> flux per volume, O<sub>2</sub> flux per mass, H<sub>2</sub>O<sub>2</sub> flux per volume) in the system do not change over time. Steady states can be measured with [[High-resolution respirometry| high-resolution respirometry]]. Combination  with  the [[TIP2k-Module| Titration-Injection  microPump (TIP2k)]] allows operation with programmable titration regimes and at quasi steady-states, yielding an expanded flexibility in experimental design  by combining the technical advantages of closed and open systems approaches.
|description=A system is in a steady state if the levels of a specific variable (e.g. O<sub>2</sub> flux, H<sub>2</sub>O<sub>2</sub> flux, mitochondrial membrane potential) in the system do not change over time. [[High-resolution respirometry| High-resolution respirometry]] allows to measure different parameters (e.g. O<sub>2</sub> flux, H<sub>2</sub>O<sub>2</sub> flux, mitochondrial membrane potential) at steady state. Combination  with  the [[TIP2k-Module| Titration-Injection  microPump (TIP2k)]] allows operation with programmable titration regimes and at quasi steady-states, yielding an expanded flexibility in experimental design  by combining the technical advantages of closed and open systems approaches.
|info=[[MiPNet06.01_O2k-Overview]], [[Harrison 2015 J Appl Physiol]]  
|info=[[MiPNet06.01_O2k-Overview]], [[Harrison 2015 J Appl Physiol]]  
}}
}}

Revision as of 07:26, 28 May 2020


high-resolution terminology - matching measurements at high-resolution


Steady state

Description

A system is in a steady state if the levels of a specific variable (e.g. O2 flux, H2O2 flux, mitochondrial membrane potential) in the system do not change over time. High-resolution respirometry allows to measure different parameters (e.g. O2 flux, H2O2 flux, mitochondrial membrane potential) at steady state. Combination with the Titration-Injection microPump (TIP2k) allows operation with programmable titration regimes and at quasi steady-states, yielding an expanded flexibility in experimental design by combining the technical advantages of closed and open systems approaches.


Reference: MiPNet06.01_O2k-Overview, Harrison 2015 J Appl Physiol


MitoPedia concepts: "MitoFit Quality Control System" is not in the list (MiP concept, Respiratory state, Respiratory control ratio, SUIT concept, SUIT protocol, SUIT A, SUIT B, SUIT C, SUIT state, Recommended, ...) of allowed values for the "MitoPedia concept" property. MitoFit Quality Control System"MitoFit Quality Control System" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 


MitoPedia methods: Respirometry, Fluorometry, Spectrophotometry 


MitoPedia O2k and high-resolution respirometry: DatLab 



Communicated by Doerrier C, 2020-04-20, updated 2020-04-23

DatLab oxygen flux: performance and data analysis

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