Difference between revisions of "Steady state"
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Communicated by [[Doerrier C]], 2020-04-20, updated 2020-04-23 | Communicated by [[Doerrier C]], 2020-04-20, updated 2020-04-23, 2020-05-28 | ||
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== DatLab oxygen flux: performance and data analysis == | == DatLab oxygen flux: performance and data analysis == | ||
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Revision as of 08:29, 28 May 2020
- high-resolution terminology - matching measurements at high-resolution
Steady state
Description
A system is in a steady state if the levels of a specific variable (e.g. O2 flux, H2O2 flux, mitochondrial membrane potential) in the system do not change over time. High-resolution respirometry allows to measure different parameters (e.g. O2 flux, H2O2 flux, mitochondrial membrane potential) at steady state. Combination with the Titration-Injection microPump (TIP2k) allows operation with programmable titration regimes and at quasi steady-states, yielding an expanded flexibility in experimental design by combining the technical advantages of closed and open systems approaches.
Reference: MiPNet06.01_O2k-Overview, Harrison 2015 J Appl Physiol
MitoPedia concepts: "MitoFit Quality Control System" is not in the list (MiP concept, Respiratory state, Respiratory control ratio, SUIT concept, SUIT protocol, SUIT A, SUIT B, SUIT C, SUIT state, Recommended, ...) of allowed values for the "MitoPedia concept" property.
MitoFit Quality Control System"MitoFit Quality Control System" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
MitoPedia methods:
Respirometry,
Fluorometry,
Spectrophotometry
MitoPedia O2k and high-resolution respirometry:
DatLab
Communicated by Doerrier C, 2020-04-20, updated 2020-04-23, 2020-05-28
