Difference between revisions of "Steinlechner-Maran 1996 Am J Physiol Cell Physiol"
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{{Publication | {{Publication | ||
|title=Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271: C2053- | |title=Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/8997208 PMID: 8997208] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/8997208 PMID: 8997208] | ||
|authors=Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E | |authors=Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E | ||
|year=1996 | |year=1996 | ||
|journal=Am J Physiol Cell Physiol | |journal=Am J Physiol Cell Physiol | ||
|abstract=We studied the oxygen dependence of respiration in cultured human umbilical vein endothelial cells by use of high-resolution respirometry. The rate of oxygen consumption varied from 30 to 50 pmol O<sub>2</sub>Β .s<sup>-1</sup>.10<sup>-6</sup> cells over a sixfold range of cell densities. Respiration was stimulated up to 3.5-fold by uncoupling with carbonyl cyanide ''p''-trifluoromethoxyphenylhydrazone or 2,4- | |abstract=We studied the oxygen dependence of respiration in cultured human umbilical vein endothelial cells by use of high-resolution respirometry. The [[ROUTINE respiration |rate of oxygen consumption]] varied from 30 to 50 pmol O<sub>2</sub>Β .s<sup>-1</sup>.10<sup>-6</sup> cells over a sixfold range of cell densities. Respiration was stimulated up to 3.5-fold by uncoupling with [[FCCP |carbonyl cyanide ''p''-trifluoromethoxyphenylhydrazone]] or 2,4-[[Dinitrophenole]], and the ''p''<sub>O2</sub> at half-maximal respiration (''p''<sub>50</sub>) increased from 0.05 to 0.12 kPa (0.3 to 0.9 Torr) with respiratory rate. ''p''<sub>50</sub> decreased to a minimum of 0.02 kPa when uncoupled cells were inhibited to control levels. Differences in cell size explained a variation of approximately 0.015 kPa in ''p''<sub>50</sub> at similar respiratory rates per cell. Oxygen diffusion to mitochondria contributed maximally 30% to the regulation of ''p''<sub>50</sub> in coupled cells, as deduced from the shallow slope of the flux dependence of ''p''<sub>50</sub> in uncoupled-inhibited cells compared with the slope in coupled cells. Therefore 70% of the flux dependence of ''p''<sub>50</sub> in coupled cells was caused by changes in metabolic state, which correlated with respiratory rate. | ||
|mipnetlab= | |mipnetlab=AT Innsbruck Gnaiger E | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
::::Β» Coupling control protocol: [[1R;2U-]] | |||
== Cited by == | |||
{{Template:Cited by Gnaiger 2021 MitoFit BCA}} | |||
{{Template:Cited by Gnaiger 2020 BEC MitoPathways}} | |||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Biogenesis;mt-density | |||
|organism=Human | |organism=Human | ||
|tissues=Endothelial; | |tissues=Endothelial;epithelial;mesothelial cell, HUVEC | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|topics=Oxygen kinetics, Uncoupler | |||
|topics= | |couplingstates=ROUTINE, ET | ||
|couplingstates=ROUTINE, | |instruments=Oxygraph-2k, O2k-Protocol | ||
|additional=SUIT-003 Ce1;ce2U-, Uncoupling, BEC 2020.2, MitoFit 2021 BCA | |||
|instruments=Oxygraph-2k | |||
| | |||
}} | }} |
Revision as of 03:45, 24 August 2021
Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. |
Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Am J Physiol Cell Physiol
Abstract: We studied the oxygen dependence of respiration in cultured human umbilical vein endothelial cells by use of high-resolution respirometry. The rate of oxygen consumption varied from 30 to 50 pmol O2 .s-1.10-6 cells over a sixfold range of cell densities. Respiration was stimulated up to 3.5-fold by uncoupling with carbonyl cyanide p-trifluoromethoxyphenylhydrazone or 2,4-Dinitrophenole, and the pO2 at half-maximal respiration (p50) increased from 0.05 to 0.12 kPa (0.3 to 0.9 Torr) with respiratory rate. p50 decreased to a minimum of 0.02 kPa when uncoupled cells were inhibited to control levels. Differences in cell size explained a variation of approximately 0.015 kPa in p50 at similar respiratory rates per cell. Oxygen diffusion to mitochondria contributed maximally 30% to the regulation of p50 in coupled cells, as deduced from the shallow slope of the flux dependence of p50 in uncoupled-inhibited cells compared with the slope in coupled cells. Therefore 70% of the flux dependence of p50 in coupled cells was caused by changes in metabolic state, which correlated with respiratory rate.
β’ O2k-Network Lab: AT Innsbruck Gnaiger E
- Β» Coupling control protocol: 1R;2U-
Cited by
- Gnaiger E (2021) Bioenergetic cluster analysis β mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.8. https://doi.org/10.26124/mitofit:2021-0008
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
Labels: MiParea: Respiration, mt-Biogenesis;mt-density
Organism: Human
Tissue;cell: Endothelial;epithelial;mesothelial cell, HUVEC
Preparation: Intact cells
Regulation: Oxygen kinetics, Uncoupler Coupling state: ROUTINE, ET
HRR: Oxygraph-2k, O2k-Protocol
SUIT-003 Ce1;ce2U-, Uncoupling, BEC 2020.2, MitoFit 2021 BCA