Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Steinlechner-Maran 1996 Am J Physiol Cell Physiol"

From Bioblast
Β 
(20 intermediate revisions by 5 users not shown)
Line 5: Line 5:
|year=1996
|year=1996
|journal=Am J Physiol Cell Physiol
|journal=Am J Physiol Cell Physiol
|abstract=We studied the oxygen dependence of respiration in cultured human umbilical vein endothelial cells by use of high-resolution respirometry. The [[ROUTINE respiration |rate of oxygen consumption]] varied from 30 to 50 pmol O<sub>2</sub>Β  .s<sup>-1</sup>.10<sup>-6</sup> cells over a sixfold range of cell densities. Respiration was stimulated up to 3.5-fold by uncoupling with [[FCCP |carbonyl cyanide ''p''-trifluoromethoxyphenylhydrazone]] or 2,4-[[dinitrophenol]], and the ''p''<sub>O2</sub> at half-maximal respiration (''p''<sub>50</sub>) increased from 0.05 to 0.12 kPa (0.3 to 0.9 Torr) with respiratory rate. ''p''<sub>50</sub> decreased to a minimum of 0.02 kPa when uncoupled cells were inhibited to control levels. Differences in cell size explained a variation of approximately 0.015 kPa in ''p''<sub>50</sub> at similar respiratory rates per cell. Oxygen diffusion to mitochondria contributed maximally 30% to the regulation of ''p''<sub>50</sub> in coupled cells, as deduced from the shallow slope of the flux dependence of ''p''<sub>50</sub> in uncoupled-inhibited cells compared with the slope in coupled cells. Therefore 70% of the flux dependence of ''p''<sub>50</sub> in coupled cells was caused by changes in metabolic state, which correlated with respiratory rate.
|abstract=We studied the oxygen dependence of respiration in cultured human umbilical vein endothelial cells by use of high-resolution respirometry. The [[ROUTINE respiration |rate of oxygen consumption]] varied from 30 to 50 pmol O<sub>2</sub>Β  .s<sup>-1</sup>.10<sup>-6</sup> cells over a sixfold range of cell densities. Respiration was stimulated up to 3.5-fold by uncoupling with [[FCCP |carbonyl cyanide ''p''-trifluoromethoxyphenylhydrazone]] or 2,4-[[Dinitrophenole]], and the ''p''<sub>O2</sub> at half-maximal respiration (''p''<sub>50</sub>) increased from 0.05 to 0.12 kPa (0.3 to 0.9 Torr) with respiratory rate. ''p''<sub>50</sub> decreased to a minimum of 0.02 kPa when uncoupled cells were inhibited to control levels. Differences in cell size explained a variation of approximately 0.015 kPa in ''p''<sub>50</sub> at similar respiratory rates per cell. Oxygen diffusion to mitochondria contributed maximally 30% to the regulation of ''p''<sub>50</sub> in coupled cells, as deduced from the shallow slope of the flux dependence of ''p''<sub>50</sub> in uncoupled-inhibited cells compared with the slope in coupled cells. Therefore 70% of the flux dependence of ''p''<sub>50</sub> in coupled cells was caused by changes in metabolic state, which correlated with respiratory rate.
|mipnetlab=AT Innsbruck Gnaiger E
|mipnetlab=AT Innsbruck Gnaiger E
|discipline=Mitochondrial Physiology
|discipline=Mitochondrial Physiology
}}
}}
::::Β» Coupling control protocol: [[1R;2U-]]
== Cited by ==
{{Template:Cited by Gnaiger 2021 MitoFit BCA}}
{{Template:Cited by Gnaiger 2020 BEC MitoPathways}}
{{Labeling
{{Labeling
|area=Respiration, mt-Biogenesis;mt-density
|area=Respiration, mt-Biogenesis;mt-density
|organism=Human
|organism=Human
|tissues=Endothelial;epithelial;mesothelial cell
|tissues=Endothelial;epithelial;mesothelial cell, HUVEC
|model cell lines=HUVEC
|preparations=Intact cells
|preparations=Intact cells
|topics=O2, Uncoupler
|topics=Oxygen kinetics, Uncoupler
|couplingstates=ROUTINE, ETS
|couplingstates=ROUTINE, ET
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k, O2k-Protocol
|discipline=Mitochondrial Physiology
|additional=SUIT-003 Ce1;ce2U-, Uncoupling, BEC 2020.2, MitoFit 2021 BCA
}}
}}

Latest revision as of 02:45, 24 August 2021

Publications in the MiPMap
Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61.

Β» PMID: 8997208

Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Am J Physiol Cell Physiol

Abstract: We studied the oxygen dependence of respiration in cultured human umbilical vein endothelial cells by use of high-resolution respirometry. The rate of oxygen consumption varied from 30 to 50 pmol O2 .s-1.10-6 cells over a sixfold range of cell densities. Respiration was stimulated up to 3.5-fold by uncoupling with carbonyl cyanide p-trifluoromethoxyphenylhydrazone or 2,4-Dinitrophenole, and the pO2 at half-maximal respiration (p50) increased from 0.05 to 0.12 kPa (0.3 to 0.9 Torr) with respiratory rate. p50 decreased to a minimum of 0.02 kPa when uncoupled cells were inhibited to control levels. Differences in cell size explained a variation of approximately 0.015 kPa in p50 at similar respiratory rates per cell. Oxygen diffusion to mitochondria contributed maximally 30% to the regulation of p50 in coupled cells, as deduced from the shallow slope of the flux dependence of p50 in uncoupled-inhibited cells compared with the slope in coupled cells. Therefore 70% of the flux dependence of p50 in coupled cells was caused by changes in metabolic state, which correlated with respiratory rate.


β€’ O2k-Network Lab: AT Innsbruck Gnaiger E

Β» Coupling control protocol: 1R;2U-

Cited by

Gnaiger E (2021) Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.8.


Gnaiger E (2021) Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.8. https://doi.org/10.26124/mitofit:2021-0008
Gnaiger 2020 BEC MitoPathways
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002



Labels: MiParea: Respiration, mt-Biogenesis;mt-density 


Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell, HUVEC  Preparation: Intact cells 

Regulation: Oxygen kinetics, Uncoupler  Coupling state: ROUTINE, ET 

HRR: Oxygraph-2k, O2k-Protocol 

SUIT-003 Ce1;ce2U-, Uncoupling, BEC 2020.2, MitoFit 2021 BCA