Steinlechner-Maran 1996 Am J Physiol Cell Physiol

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Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61.

» PMID: 8997208

Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Am J Physiol Cell Physiol

Abstract: We studied the oxygen dependence of respiration in cultured human umbilical vein endothelial cells by use of high-resolution respirometry. The rate of oxygen consumption varied from 30 to 50 pmol O₂ .s^-1.10^-6 cells over a sixfold range of cell densities. Respiration was stimulated up to 3.5-fold by uncoupling with carbonyl cyanide p-trifluoromethoxyphenylhydrazone or 2,4-Dinitrophenole, and the p_O2 at half-maximal respiration (p₅₀) increased from 0.05 to 0.12 kPa (0.3 to 0.9 Torr) with respiratory rate. p₅₀ decreased to a minimum of 0.02 kPa when uncoupled cells were inhibited to control levels. Differences in cell size explained a variation of approximately 0.015 kPa in p₅₀ at similar respiratory rates per cell. Oxygen diffusion to mitochondria contributed maximally 30% to the regulation of p₅₀ in coupled cells, as deduced from the shallow slope of the flux dependence of p₅₀ in uncoupled-inhibited cells compared with the slope in coupled cells. Therefore 70% of the flux dependence of p₅₀ in coupled cells was caused by changes in metabolic state, which correlated with respiratory rate.

• O2k-Network Lab: AT Innsbruck Gnaiger E

» Coupling control protocol: 1R;2U-

Cited by

Gnaiger E (2021) Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.8. https://doi.org/10.26124/mitofit:2021-0008

Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5^th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002

Labels: MiParea: Respiration, mt-Biogenesis;mt-density

Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell, HUVEC Preparation: Intact cells

Regulation: Oxygen kinetics, Uncoupler Coupling state: ROUTINE, ET

HRR: Oxygraph-2k, O2k-Protocol

SUIT-003 Ce1;ce2U-, Uncoupling, BEC 2020.2, MitoFit 2021 BCA