Difference between revisions of "Substrate-uncoupler-inhibitor titration"
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==SUIT protocols== | ==SUIT protocols== | ||
:::: SUIT protocols have been developed for the first time on the basis of [[ | :::: SUIT protocols have been developed for the first time on the basis of [[High-Resolution FluoRespirometry]], since their application depends critically on long-term stability of the [[polarographic oxygen sensor]] (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: [[Gnaiger_2000_MitoInTheCold]]; [[MiR06]]) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature. | ||
::: Attempts are in progress to develop a database of SUIT protocols | ::: Attempts are in progress to develop a database of SUIT protocols | ||
Revision as of 13:35, 22 January 2018
- high-resolution terminology - matching measurements at high-resolution
Substrate-uncoupler-inhibitor titration
Description
Mitochondrial Substrate-uncoupler-inhibitor titration (SUIT) protocols are used with mitochondrial preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental assay.
Abbreviation: SUIT
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MitoPedia: SUIT
MitoPedia concepts:
MiP concept,
SUIT concept
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor,
Substrate and metabolite,
Uncoupler
Contributed by Gnaiger E 2010-09-18, edited 2016-01-24.
SUIT protocols
- SUIT protocols have been developed for the first time on the basis of High-Resolution FluoRespirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.
- Attempts are in progress to develop a database of SUIT protocols
