Difference between revisions of "Substrate-uncoupler-inhibitor titration"

Latest revision as of 08:57, 27 May 2020

high-resolution terminology - matching measurements at high-resolution

Substrate-uncoupler-inhibitor titration

Description

Mitochondrial Substrate-uncoupler-inhibitor titration (SUIT) protocols are used with mitochondrial preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental assay.

Abbreviation: SUIT

Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MitoPedia: SUIT

Contributed by Gnaiger E 2010-09-18, edited 2016-01-24.

SUIT protocols

SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.

Attempts are in progress to develop a database of SUIT protocols

References

Bioblast link	Reference	Year
BEC 2020.1 doi10.26124bec2020-0001.v1	Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1	2020

Boushel 2007 Diabetologia
Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - »Bioblast link«
Gnaiger 2007 MitoPathways » new edition: Gnaiger 2014 MitoPathways
Gnaiger 2009 Int J Biochem Cell Biol
Lemieux 2011 Int J Biochem Cell Biol
Pesta 2012 Methods Mol Biol
Pesta 2011 Am J Physiol Regul Integr Comp Physiol
Sjoevall 2010 Crit Care

MitoPedia concepts: MiP concept, SUIT concept

MitoPedia methods: Respirometry

MitoPedia topics: Inhibitor, Substrate and metabolite, Uncoupler

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=SUIT
-|description='''SUIT''' protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations.
+|description=Mitochondrial '''Substrate-uncoupler-inhibitor titration''' ('''SUIT''') [[MitoPedia: SUIT |protocols]] are used with [[mitochondrial preparations]] to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental [[assay]].
-|info=[[MiPNet12.12]], [[MiPNet09.12]], [[Gnaiger_2007_MitoPathways]], [[Gnaiger_2009_IJCBC]]
+|info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MitoPedia: SUIT]]
-|type=Respiration
 }}
-{{Labeling
-|discipline=Mitochondrial Physiology
-|preparations=Isolated Mitochondria, Permeabilized Cell or Tissue; Homogenate
+Contributed by [[Gnaiger E]] 2010-09-18, edited 2016-01-24.
-|kinetics=Inhibitor; Uncoupler
-|topics=Respiration; OXPHOS; ETS Capacity, Flux Control; Additivity; Threshold; Excess Capacity, Coupling; Membrane Potential, Substrate; Glucose; TCA Cycle, Fatty Acid, Amino Acid, ATP; ADP; AMP; PCr
+[[File:Pesta_2012_Methods_Mol_Biol_Fig7A.jpg|right|500px|link=Pesta_2012_Methods_Mol_Biol|Pesta and Gnaiger 2012]]
+==SUIT protocols==
+:::: SUIT protocols have been developed for the first time on the basis of [[high-resolution respirometry]], since their application depends critically on long-term stability of the [[polarographic oxygen sensor]] (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: [[Gnaiger_2000_MitoInTheCold]]; [[MiR06]]) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.
+::: Attempts are in progress to develop a database of SUIT protocols
+::::* [[SUIT protocol library]]
+::::* [[MitoPedia:_SUIT#SUIT_protocols |MitoPedia: SUIT protocols]]
+== References ==
+{{#ask:[[Additional label::Substrate-uncoupler-inhibitor titration]]
+| mainlabel=Bioblast link
+|?Has title=Reference
+|?Was published in year=Year
+|format=broadtable
+|limit=5000
+|offset=0
+|sort=Has title
+|order=ascending
+}}
+{{#ask:[[Additional label::SUIT]]
+| mainlabel=Bioblast link
+|?Has title=Reference
+|?Was published in year=Year
+|format=broadtable
+|limit=5000
+|offset=0
+|sort=Has title
+|order=ascending
+}}
+::::* [[Boushel 2007 Diabetologia]]
+::::* Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - [[Doerrier 2018 Methods Mol Biol |»Bioblast link«]]
+::::* [[Gnaiger 2007 MitoPathways]] » ''new edition:'' [[Gnaiger 2014 MitoPathways]]
+::::* [[Gnaiger 2009 Int J Biochem Cell Biol]]
+::::* [[Lemieux 2011 Int J Biochem Cell Biol]]
+::::* [[Pesta 2012 Methods Mol Biol]]
+::::* [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol]]
+::::* [[Sjoevall 2010 Crit Care]]
+{{MitoPedia concepts
+|mitopedia concept=MiP concept, SUIT concept
+}}
+{{MitoPedia methods
+|mitopedia method=Respirometry
+}}
+{{MitoPedia topics
+|mitopedia topic=Inhibitor, Substrate and metabolite, Uncoupler
 }}
-SUIT protocols have been developed for the first time on the basis of [[high-resolution respirometry]], since their application depends critically on long-term stability of the [[polarographic oxygen sensor]] (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: [[Gnaiger_2000_MitoInTheCold]]; [[MiR06]]) have been developed to maintain respiratory capacity during application of SUIT protocols at physiological temperature ([[Gnaiger_2007_MitoPathways]]).