- high-resolution terminology - matching measurements at high-resolution
Succinate
Description
Succinate2- is formed in the TCA cycle, and is a substrate of CII, reacting to fumarate and feeding electrons into the Q-junction through flavin adenine dinucleotide (FADH2). Succinate supports electron flux exclusively through Complex II via FADH2.
Abbreviation: S
Reference: MiPNet11.09, MiPNet12.11
Labels:
Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
HRR
S: Succinate disodium salt, hexahydrate, C4H4O4Na2.6H2O; FW=270.1.
Stock solution: 1.000 mM (1 M; 2.701 g/10 ml H2O); pH should be 7.0 - check with pH paper and adjust if necessary. Divide into 0.5 ml portions. Store frozen at -20 °C. Sigma, S 2378 (100 g) RT. MiPNet03.02
Final conc. in 2 ml O2k-chamber: 10 mM; titrate 20 µl using the 50 µl syringe. MiPNet09.12
