Superoxide dismutase: Difference between revisions

Revision as of 13:42, 17 March 2019

high-resolution terminology - matching measurements at high-resolution

Superoxide dismutase

Description

Mammalian superoxide dismutase (SOD) exists in three forms, of which the Mn-SOD occurs in mitochondria (mtSOD, SOD2; 93 kD homotetramer) and many bacteria, in contrast to the Cu-Zn forms of SOD (cytosolic SOD1, extracellular SOD3 anchored to the extracellular matrix and cell surface). Superoxide anion (O₂^•-) is a major reactive oxygen species (ROS) which is dismutated by SOD to oxygen and H₂O₂.

Abbreviation: SOD

Reference: Fridovich_1997_J Biol Chem

MitoPedia topics: Enzyme

Application in HRR

Add SOD for conversion of O₂^•- and measurement of H₂O₂ with Amplex ultrared.
SOD: Superoxide dismutase from bovine liver, Sigma S8160, 15 KU, store at -20°C; MW = 32.000,00 g/mol

Preparation of 5000 U/mL stock solution (dissolved in H₂O)

Weigh 2 mg of superoxide dismutase.
Dissolve in 1134 µL H₂O.
Divide into 30 µL portions into 0.2 mL Eppendorf tubes.
Store frozen at -20 °C in the desiccator.

@@ Line 8: / Line 8: @@
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 [[File:O2-.jpg|right|60px|Superoxide anion]]
-== Application in [[HRFR]] ==
+== Application in [[HRR]] ==
 ::::* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[MiPNet20.14_AmplexRed_H2O2-production|measurement of H<sub>2</sub>O<sub>2</sub> with Amplex ultrared]].
 ::::* '''SOD: Superoxide dismutase from bovine liver''', Sigma S8160, 15 KU, store at -20°C; MW = 32.000,00 g/mol
-::: '''Preparation of 5000 U/ml stock solution''' (dissolved in H<sub>2</sub>O)
+::: '''Preparation of 5000 U/mL stock solution''' (dissolved in H<sub>2</sub>O)
 ::::# Weigh 2 mg of superoxide dismutase.
-::::# Dissolve in 1134 µl H<sub>2</sub>O.
+::::# Dissolve in 1134 µL H<sub>2</sub>O.
-::::# Divide into 30 µl portions into 0.2 ml Eppendorf tubes.
+::::# Divide into 30 µL portions into 0.2 mL Eppendorf tubes.
 ::::# Store frozen at -20 °C in the desiccator.