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Former Instructions
These preparation instructions are still correct. Ordering ADP in portions of 1 g per vial turned out to be very useful and is therefore the recommended strategy (as described on the ADP main page).
Preparation without MgCl2 and lower amounts of ADP
- Preparation of 500 mM ADP stock solution (dissolved in H2O):
- Weigh 501.3 mg of ADP = 1 mmol ADP
- Add 1.2 mL H2O (ADP is not dissolved at this stage).
- Neutralize with 5 M KOH (approx. 450 ยตL). ADP will dissolve after addition of KOH.
- Check pH and adjust to 7 with 5 M KOH if necessary.
- Adjust final volume to 2 mL and divide into 0.2 mL portions.
- Store at -80 ยฐC.
Preparation with MgCl2 and lower amounts of ADP
- Preparation of 500 mM ADP stock solution with 300 mM free Mg2+ (dissolved in H2O):
- Weigh 501.3 mg of ADP = 1 mmol ADP.
- Add 1.2 mL H2O (ADP is not dissolved at this stage).
- Neutralize with 5 M KOH (approx. 450 ยตl). ADP will dissolve after addition of KOH.
- Add 121.98 mg MgCl2. White precipitate will occur, which will dissolve during 1-2 min stirring on magnetic stirrer at RT.
- Check pH and adjust to 7 with 5 M KOH if necessary.
- Adjust final volume to 2 mL and divide into 0.2 mL portions.
- Store at -80 ยฐC.