Talk:Cardoso Luiza HD: Difference between revisions
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::: [[Cardoso LHD]] has been accessed more than | ::: [[Cardoso LHD]] has been accessed more than | ||
::::* 5,000 times (2019-12-16) | ::::* 5,000 times (2019-12-16) | ||
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] Do you know how to evaluate '''fatty acid oxidation''' avoiding to overestimate it?''' | |||
:::::::- Did you know that '''malate alone might support mt-respiration''', e.g. if the [[Malic enzyme| mt-malic enzyme]] is present in [[Mitochondrial preparations| mitochondrial preparations]]? Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''« | |||
:::::::- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''« | |||
:::::::- [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions. '''SUIT-002''' is specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''« | |||
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] How can I measure '''ATP production''' in my '''cultured cells'''? | |||
:::::::- Measuring respiration of living cells will provide you important parameters, but calling it ATP production is not correct - »'''[[Magnesium_Green#Is_it_possible_to_measure_ATP_production_in_the_protocol_SUIT-003_O2_ce_D009.3F|SUIT protocols to measure ATP production]]'''« | |||
:::::::- Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green - »'''[[Magnesium_Green|Magnesium Green]]'''« | |||
Revision as of 11:11, 30 April 2020
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- Cardoso LHD has been accessed more than
- 5,000 times (2019-12-16)
- Cardoso LHD has been accessed more than
Do you know how to evaluate fatty acid oxidation avoiding to overestimate it?
- - Did you know that malate alone might support mt-respiration, e.g. if the mt-malic enzyme is present in mitochondrial preparations? Learn more about - »malate anaplerotic pathway control state«
- - DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«
- - F-pathway can be overestimated due to anaplerotic reactions. SUIT-002 is specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«
- How can I measure ATP production in my cultured cells?
- - Measuring respiration of living cells will provide you important parameters, but calling it ATP production is not correct - »SUIT protocols to measure ATP production«
- - Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green - »Magnesium Green«
