Difference between revisions of "Talk:MiPMap"
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== Comments on MiPMap == | |||
* ''Good concept regarding the [[MiPMap]] -- it could save lots of time and effort on the part of many investigators, not only by avoiding many hours of traditional search engine work, but also avoiding 're-inventing the wheel' in terms of experimental approaches.'' [[Hand SC]] (2012-12-26) | |||
== Example == | |||
In the MiPMap you would find information on '''mouse''' cell lines and tissues, e.g. '''mouse kidney mitochondria''': | |||
:: * Is malic enzyme present? | |||
:: * Is the ''P/E''<1.0 like in mouse brain but different from mouse skeletal muscle? | |||
:: * Which UCPs are present? | |||
:: * Preparation of isolated mitochondria, permeabilized tissue, homogenate. | |||
:: * Which respiration medium is recommended? ->> Do not use media with high K<sup>+</sup>, which inhibits kidney mitochondrial respiration. | |||
>> Is this a general issue for kidney, or is it in addition also a species issue? (according to [[Calzia E]] MiR05 works fine with kidney tissue from pig, but not in mouse). | |||
>> [[MiPNet14.13_Medium-MiR06#Limitations_of_using_MiR05_to_MiR06Cr|Limitations of using MiR05 to MiR06Cr]] | |||
Revision as of 11:31, 31 October 2013
Comments on MiPMap
- Good concept regarding the MiPMap -- it could save lots of time and effort on the part of many investigators, not only by avoiding many hours of traditional search engine work, but also avoiding 're-inventing the wheel' in terms of experimental approaches. Hand SC (2012-12-26)
Example
In the MiPMap you would find information on mouse cell lines and tissues, e.g. mouse kidney mitochondria:
- * Is malic enzyme present?
- * Is the P/E<1.0 like in mouse brain but different from mouse skeletal muscle?
- * Which UCPs are present?
- * Preparation of isolated mitochondria, permeabilized tissue, homogenate.
- * Which respiration medium is recommended? ->> Do not use media with high K+, which inhibits kidney mitochondrial respiration.
>> Is this a general issue for kidney, or is it in addition also a species issue? (according to Calzia E MiR05 works fine with kidney tissue from pig, but not in mouse).
