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| == Popular Bioblast page == | | == Popular Bioblast page == |
| ::: [[MiPNet20.14 AmplexRed H2O2-production]] has been accessed more than | | ::: [[MiPNet20.14 AmplexRed H2O2-production]] has been accessed more than |
| | ::::* 10,000 times (2019-12-12) |
| ::::* 5,000 times (2016-06-29) | | ::::* 5,000 times (2016-06-29) |
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| [[High-resolution respirometry]] is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: [[O2k-Publications:_O2k-Fluorometry_-_Fluorophores|H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels]].
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| Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
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| # Quality control of preparations by respirometric performance.
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| # Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores.
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| # Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
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| # Additional information is acquired for a limited amount of sample.
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| # Oxygen dependence of parameters can be studied.
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| In the publication by [[Krumschnabel_2015_Methods_Mol_Biol|Krumschnabel et al. 2015]] we introduce the O2k-Fluo LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H<sub>2</sub>O<sub>2</sub> production of mouse brain mitochondria. The paper by [[Makrecka-Kuka_2015_Biomolecules|Makrecka-Kuka et al. 2015]] focuses on the application of the Amplex red assay for determining H<sub>2</sub>O<sub>2</sub> production in three different models of mitochondrial preparations.
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| {{Keywords H2O2}}
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