Difference between revisions of "Talk:O2k-FluoRespirometer"

Revision as of 09:36, 5 June 2019

Talk:O2k-FluoRespirometer

Description	The Oroboros O2k-FluoRespirometer (O2k-Series H) - the experimental system complete for high-resolution respirometry (HRR), including fluorometry and the TIP2k, allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca²⁺ (CaG) or ATP production (MgG). The O2k-FluoRespirometer supports all add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module, enabling measurement of mt-membrane potential with ion sensitive electrode (ISE for TPP+ or TPMP+ ) or pH. Parameters: oxygen consumption ROS production ATP production Ca2+ pH mitochondrial membrane potential An additional OroboPOS sowie * MiR05-Kit sind included in the delivery of this O2k-Package. The O2k is a sole source apparatus with no other instruments meeting its specifications.
Product ID	10002-02
Type	O2k
Link	Oroboros O2k, O2k-FluoRespirometer manual
Image

The O2k-FluoRespirometer^[1] does not include, but fully supports add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module.

↑ A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.

O2k-Series H: innovations

O2k-Series H: innovations

Oroboros (2017-07-11) Mitochondr Physiol Network

Abstract: Mészáros A, Krumschnabel G, Gradl P, Schwaninger H, Gnaiger E (2017) O2k-Series H: innovations. Mitochondr Physiol Network 22.09

O2k-Series H is the latest version of the Oroboros O2k for high-resolution respirometry and O2k-MultiSensor applications. The O2k-Series H sets a new standard in merging high-end technology and corporate social responsibility.

• O2k-Network Lab: AT_Innsbruck_Oroboros

Features: integrated FluoRespirometry

The Fluorescence-control unit is integrated into the O2k-Main Unit. The new O2k-Window Frame is optimized for fixation of the Fluorescence-Sensors. The previous Window Tool, Front Fixation and Sensor-Guide are not required.
Smart Fluo-Sensors are pre-calibrated with sensor-specific memory and direct input into DatLab 7. O2k-Series A to G do not support the Smart Fluo-Sensors.

Features of O2k-Series H:

Fluo-Control Unit integrated into O2k-Main Unit.
Smart Fluo-Sensors with pre-calibrated light intensities for direct input into DatLab 7

DL-Protocols as real-time guides for titrations in DatLab 7, context-sensitive help. Further details » MitoPedia: DatLab.

New: DatLab 7

DatLab 7 with context-sensitive help.
DL-Protocols as real-time guides for titrations in DatLab 7, context-sensitive help. Further details » MitoPedia: DatLab.

Electronic innovations

High-resolution A-D converter: The new 24 bit analogue-digital converters provide a higher precision in measurement of O2, Amp-signals (amperometric fluorometry and nitric oxide measurements), and pX-signals (potentiometric measurements).

Core Relative Humidity and Core Relative Temperature: In addition to the built-in ambient temperature sensor, two new measurement channels are implemented to continuously monitor conditions within the electronic Main-Unit. Such data are especially important for quality control of experiments performed at high humidity and varying external temperature.

A 16 GB, industry-rated flash drive is integrated for storage of calibration files and other instrument-specific data. (This function will be available upon release of a later version of DatLab).

USB 2.0 Hi-Speed hub (4 external ports) for additional USB devices.

Three internal USB ports allow further upgrades and upwards compatibility with new developments.

Increased energy efficiency based on the new O2k-electronics design: power consumption is 24 W at 37 °C (80% reduction compared to Series D). Running costs are substantially lower as a result of our general concept for sustainable technology. The maximal power input is 120 W.

O2k net weight is reduced to 13.5 kg in comparison to 21 kg in Series D.

Previous O2k-Series D-G Product ID 10010-01

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[1] A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.

[1]

@@ Line 1: / Line 1: @@
+{{Product
+|description=The Oroboros '''O2k-FluoRespirometer''' ([[O2k-FluoRespirometer#O2k-Series_H:_innovations |O2k-Series H]]) - the experimental system complete for [[high-resolution respirometry]] (HRR), including fluorometry and the [[TIP2k-Module |TIP2k]], allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca<sup>2+</sup> (CaG) or ATP production (MgG).
+The O2k-FluoRespirometer supports all add-on [[O2k-Catalogue: O2k-Modules |O2k-Modules]]: [[O2k-TPP+ ISE-Module]], [[O2k-pH ISE-Module]], [[O2k-NO Amp-Module]], enabling measurement of mt-membrane potential with ion sensitive electrode (ISE for TPP+ or TPMP+ ) or pH.
+'''Parameters:'''
+* oxygen consumption
+* ROS production
+* ATP production
+* Ca2+
+* pH
+* mitochondrial membrane potential
+An additional OroboPOS sowie * [[MiR05-Kit]] sind included in the delivery of this O2k-Package.
+The O2k is a sole source apparatus with no other instruments meeting its [[MiPNet06.05 Specifications | specifications]].
+|product id=10002-02
+|product type=O2k
+|info=[[Oroboros O2k]], [[MiPNet22.11 O2k-FluoRespirometer manual| O2k-FluoRespirometer manual]]
+|product image=[[Image:O2k-Fluorometer.jpg|200px|right|O2k-FluoRespirometer]]
+}}
+[[Image:O2k-FluoRespirometer Concept.jpg|600px|right|O2k-FluoRespirometer]]
+__TOC__
+:::: The '''O2k-FluoRespirometer'''<ref>A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see [[DatLab#PC requirements| here]]. </ref> does not include, but fully supports add-on O2k-Modules: [[O2k-TPP+ ISE-Module]], [[O2k-pH ISE-Module]], [[O2k-NO Amp-Module]].
+<references/>
+== '''O2k-Series H: innovations'''  ==
+{{Publication
+|title=[[Image:O2k-Info.png|right|60px|link=About Oroboros|About Oroboros]]  O2k-Series H: innovations
+|authors=Oroboros
+|year=2017-07-11
+|journal=Mitochondr Physiol Network
+|abstract='''Mészáros A, Krumschnabel G, Gradl P, Schwaninger H, Gnaiger E (2017) O2k-Series H: innovations. Mitochondr Physiol Network 22.09'''
+'''O2k-Series H''' is the latest version of the Oroboros O2k for high-resolution respirometry and O2k-MultiSensor applications. The O2k-Series H sets a new standard in merging high-end technology and corporate social responsibility.
+|mipnetlab=AT_Innsbruck_Oroboros
+}}
+=== Features: integrated FluoRespirometry ===
+::::* The Fluorescence-control unit is '''integrated''' into the O2k-Main Unit. The new O2k-Window Frame is optimized for fixation of the Fluorescence-Sensors. The previous Window Tool, Front Fixation and Sensor-Guide are not required.
+::::* '''[[Smart Fluo-Sensor]]s are pre-calibrated''' with sensor-specific memory and direct input into DatLab 7. O2k-Series A to G do not support the Smart Fluo-Sensors.
+'''Features of O2k-Series H:'''
+* Fluo-Control Unit integrated into O2k-Main Unit.
+* [[Smart Fluo-Sensor]]s with pre-calibrated light intensities for direct input into DatLab 7
+* DL-Protocols as real-time guides for titrations in DatLab 7, context-sensitive help. ''Further details'' » [[MitoPedia: DatLab]].
+=== New: DatLab 7 ===
+::::* [[DatLab]] 7 with context-sensitive help.
+::::* DL-Protocols as real-time guides for titrations in DatLab 7, context-sensitive help. ''Further details'' » [[MitoPedia: DatLab]].
+=== Electronic innovations ===
+::::* '''High-resolution A-D converter:''' The new 24 bit analogue-digital converters provide a higher precision in measurement of O2, Amp-signals (amperometric fluorometry and nitric oxide measurements), and pX-signals (potentiometric measurements).
+::::* '''Core Relative Humidity''' and '''Core Relative Temperature''': In addition to the built-in ambient temperature sensor, two new measurement channels are implemented to continuously monitor conditions within the electronic Main-Unit. Such data are especially important for quality control of experiments performed at high humidity and varying external temperature.
+::::* A 16 GB, industry-rated '''flash drive is integrated''' for storage of calibration files and other instrument-specific data. (This function will be available upon release of a later version of DatLab).
+::::* '''USB 2.0 Hi-Speed hub''' (4 external ports) for additional USB devices.
+::::* '''Three internal USB ports''' allow further upgrades and upwards compatibility with new developments.
+::::* '''Increased energy efficiency''' based on the new O2k-electronics design: power consumption is 24 W at 37 °C (80% reduction compared to Series D). Running costs are substantially lower as a result of our general concept for sustainable technology. The maximal power input is 120 W.
+::::* O2k '''net weight is reduced''' to 13.5 kg in comparison to 21 kg in Series D.
 Previous O2k-Series D-G Product ID 10010-01