|
|
| Line 1: |
Line 1: |
|
| |
|
| {{Product
| |
| |description=The Oroboros '''O2k-FluoRespirometer''' ([[O2k-FluoRespirometer#O2k-Series_H:_innovations |O2k-Series H]]) - the experimental system complete for [[high-resolution respirometry]] (HRR), including fluorometry and the [[TIP2k-Module |TIP2k]], allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca<sup>2+</sup> (CaG) or ATP production (MgG). An additional OroboPOS and [[MiR05-Kit]] are included in the delivery of this O2k-Package.
| |
|
| |
| The O2k-FluoRespirometer supports all add-on [[O2k-Catalogue: O2k-Modules |O2k-Modules]]: [[O2k-TPP+ ISE-Module]], [[O2k-pH ISE-Module]], [[O2k-NO Amp-Module]], enabling measurement of mt-membrane potential with ion sensitive electrode (ISE for TPP+ or TPMP+ ) or pH.
| |
|
| |
|
| |
| '''Parameters:'''
| |
| * Oxygen consumption
| |
| * ROS production
| |
| * ATP production
| |
| * Ca<sup>2+</sup>
| |
| * pH
| |
| * Mitochondrial membrane potential
| |
|
| |
|
| |
|
| |
| The O2k is a sole source apparatus with no other instruments meeting its [[MiPNet06.05 Test Experiments on O2k-Specifications | specifications]].
| |
| |product id=10002-02
| |
| |product type=O2k
| |
| |info=[[Oroboros O2k]], [[MiPNet22.11 O2k-FluoRespirometer manual| O2k-FluoRespirometer manual]]
| |
| |product image=[[Image:O2k-Fluorometer.jpg|200px|right|O2k-FluoRespirometer]]
| |
| }}
| |
| [[Image:O2k-FluoRespirometer Concept.jpg|600px|right|O2k-FluoRespirometer]]
| |
| __TOC__
| |
| :::: The '''O2k-FluoRespirometer'''<ref>A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see [[DatLab#PC requirements| here]]. </ref> does not include, but fully supports add-on O2k-Modules: [[O2k-TPP+ ISE-Module]], [[O2k-pH ISE-Module]], [[O2k-NO Amp-Module]].
| |
| <references/>
| |
| == '''O2k-Series H: innovations''' ==
| |
| {{Publication
| |
| |title=[[Image:O2k-Info.png|right|60px|link=About Oroboros|About Oroboros]] O2k-Series H: innovations
| |
| |authors=Oroboros
| |
| |year=2017-07-11
| |
| |journal=Mitochondr Physiol Network
| |
| |abstract='''Mészáros A, Krumschnabel G, Gradl P, Schwaninger H, Gnaiger E (2017) O2k-Series H: innovations. Mitochondr Physiol Network 22.09'''
| |
|
| |
| '''O2k-Series H''' is the latest version of the Oroboros O2k for high-resolution respirometry and O2k-MultiSensor applications. The O2k-Series H sets a new standard in merging high-end technology and corporate social responsibility.
| |
| |mipnetlab=AT_Innsbruck_Oroboros
| |
| }}
| |
|
| |
| === Features: integrated FluoRespirometry ===
| |
| ::::* The Fluorescence-control unit is '''integrated''' into the O2k-Main Unit. The new O2k-Window Frame is optimized for fixation of the Fluorescence-Sensors. The previous Window Tool, Front Fixation and Sensor-Guide are not required.
| |
| ::::* '''[[Smart Fluo-Sensor]]s are pre-calibrated''' with sensor-specific memory and direct input into DatLab 7. O2k-Series A to G do not support the Smart Fluo-Sensors
| |
|
| |
| === New: DatLab 7 ===
| |
| ::::* [[DatLab]] 7 with context-sensitive help.
| |
| ::::* DL-Protocols as real-time guides for titrations in DatLab 7, context-sensitive help. ''Further details'' » [[MitoPedia: DatLab]].
| |
|
| |
| === Electronic innovations ===
| |
| ::::* '''High-resolution A-D converter:''' The new 24 bit analogue-digital converters provide a higher precision in measurement of O2, Amp-signals (amperometric fluorometry and nitric oxide measurements), and pX-signals (potentiometric measurements).
| |
|
| |
| ::::* '''Core Relative Humidity''' and '''Core Relative Temperature''': In addition to the built-in ambient temperature sensor, two new measurement channels are implemented to continuously monitor conditions within the electronic Main-Unit. Such data are especially important for quality control of experiments performed at high humidity and varying external temperature.
| |
|
| |
| ::::* A 16 GB, industry-rated '''flash drive is integrated''' for storage of calibration files and other instrument-specific data. (This function will be available upon release of a later version of DatLab).
| |
|
| |
| ::::* '''USB 2.0 Hi-Speed hub''' (4 external ports) for additional USB devices.
| |
|
| |
| ::::* '''Three internal USB ports''' allow further upgrades and upwards compatibility with new developments.
| |
|
| |
| ::::* '''Increased energy efficiency''' based on the new O2k-electronics design: power consumption is 24 W at 37 °C (80% reduction compared to Series D). Running costs are substantially lower as a result of our general concept for sustainable technology. The maximal power input is 120 W.
| |
|
| |
| ::::* O2k '''net weight is reduced''' to 13.5 kg in comparison to 21 kg in Series D.
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
| Previous O2k-Series D-G Product ID 10010-01
| |
|
| |
|
| |
| [[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]]
| |
| == Popular Bioblast page ==
| |
| ::: [[O2k-FluoRespirometer]] has been accessed more than
| |
| ::::* 20,000 times (2019-07-22)
| |
| ::::* 15,000 times (2019-03-13)
| |
| ::::* 10,000 times (2017-07-18)
| |
