Talk:O2k-Open Support alert: Difference between revisions
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?''' | ||
::::::: While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - Β»[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]Β« | ::::::: While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - Β»[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]Β« | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Did you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background on the [[O2k-sV-Module|Small O2k-Chamber]] (0.5 mL)?''' | |||
::::::: By adjusting the dithionite concentration toΒ 2.5 mM, instrumental O2 background can be performed with the standard settings on the [[O2k-sV-Module|Small O2k-Chamber]] (0.5 mL).Β ''Further details in'' [[Dithionite]] and Β»[[MiPNet14.06 Instrumental O2 background]] |
Revision as of 09:49, 5 March 2020
- Do you perform the leaky chamber test after chamber assembly?
- After chamber reassembly you might experience a small leaky chamber only identified by a leaky chamber test- Β»Leaky chamber testΒ«
- Do you perform the leaky chamber test after chamber assembly?
- Do you change the slope smoothing to 20 while running experiments with biological sample?
- While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - Β»Slope smoothingΒ«
- Do you change the slope smoothing to 20 while running experiments with biological sample?
- Did you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the Small O2k-Chamber (0.5 mL)?
- By adjusting the dithionite concentration to 2.5 mM, instrumental O2 background can be performed with the standard settings on the Small O2k-Chamber (0.5 mL). Further details in Dithionite and Β»MiPNet14.06 Instrumental O2 background
- Did you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the Small O2k-Chamber (0.5 mL)?