Difference between revisions of "Talk:O2k-Open Support alert"
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== In discussion for page == | |||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform the ''leaky chamber test'' after chamber assembly? ''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform the ''leaky chamber test'' after chamber assembly? ''' | ||
::::::: After chamber reassembly you might experience a small leaky chamber only identified by a ''leaky chamber test''- »[[O2k-Chamber_MitoPedia#Leaky_chamber_test|Leaky chamber test]]« | ::::::: After chamber reassembly you might experience a small leaky chamber only identified by a ''leaky chamber test''- »[[O2k-Chamber_MitoPedia#Leaky_chamber_test|Leaky chamber test]]« | ||
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?''' | ||
::::::: See how to clean and dry the electrical connections of the OroboPOS-Connector.-»[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]« | ::::::: See how to clean and dry the electrical connections of the OroboPOS-Connector.-»[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]« | ||
== O2k-Open Support alert circulars == | |||
=== O2k-Open Support alert circular 2020(01): in prep === | |||
::'''''New''''' [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.''' | |||
::::::: See how you can check that the two blue OroboPOS-Holders are intact. - »[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]« | |||
<br /> | |||
::'''''New''''' [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Calibration parameters are transferred to experimental DatLab files''' | |||
::::::: Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]« | |||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?''' | |||
::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). - »[[O2k-Chamber MitoPedia]]« | |||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab? | |||
::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. - »[[Oxygen solubility factor]]« |
Revision as of 14:44, 10 March 2020
In discussion for page
- After chamber reassembly you might experience a small leaky chamber only identified by a leaky chamber test- »Leaky chamber test«
- Do you change the slope smoothing to 20 while running experiments with biological sample?
- While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »Slope smoothing«
- Do you change the slope smoothing to 20 while running experiments with biological sample?
- Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the O2k-sV-Module?
- By adjusting the dithionite concentration to 2.5 mM, the instrumental O2 background can be performed with specific settings for the O2k-sV-Module. - »O2k-sV-Module«
- Do you know how the "switch components" approach can guide you to find what is causing your O2k not to reach the required specifications?
- Do not desperate if specifications given in the O2k-Manuals are not obtained. See how to perform an experimental troubleshooting on your own.-»Switch components approach«
- Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
- See how to clean and dry the electrical connections of the OroboPOS-Connector.-»Cleaning the electrical connections«
- Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the O2k-sV-Module?
O2k-Open Support alert circulars
O2k-Open Support alert circular 2020(01): in prep
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- New Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
- New Calibration parameters are transferred to experimental DatLab files
- The O2 solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«