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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform the ''leaky chamber test'' after chamber assembly? '''
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| ::::::: After chamber reassembly you might experience a small leaky chamber only identified by a ''leaky chamber test''- »[https://www.bioblast.at/index.php/O2k-Chamber_MitoPedia#Leaky_chamber_test Leaky chamber test]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?'''
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| ::::::: While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Did you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background on the [[O2k-sV-Module|Small O2k-Chamber]] (0.5 mL)?'''
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| ::::::: By adjusting the dithionite concentration to 2.5 mM, instrumental O2 background can be performed with the standard settings on the [[O2k-sV-Module|Small O2k-Chamber]] (0.5 mL). ''Further details in'' [[Dithionite]] and »[[MiPNet14.06 Instrumental O2 background]]
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