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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform the ''leaky chamber test'' after chamber assembly? '''
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| ::::::: After chamber reassembly you might experience a small leaky chamber only identified by a ''leaky chamber test''- »[[O2k-Chamber_MitoPedia#Leaky_chamber_test|Leaky chamber test]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?'''
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| ::::::: While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background on the [[O2k-sV-Module]]?'''
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| ::::::: By adjusting the [[Dithionite|dithionite]] concentration to 2.5 mM, the [[MiPNet14.06 Instrumental O2 background|instrumental O<sub>2</sub> background]] can be performed with specific settings for the O2k-sV-Module. - »[[O2k-sV-Module]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know how the "switch components" approach can guide you to find what is causing your O2k not to reach the required specifications?'''
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| ::::::: Do not desperate if specifications given in the O2k-Manuals are not obtained. See how to perform an experimental troubleshooting on your own.-»[[Oxygen_sensor_test#Switch_components_approach|Switch components approach]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?'''
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| ::::::: See how to clean and dry the electrical connections of the OroboPOS-Connector.-»[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]«
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