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Difference between revisions of "Talk:O2k-Open Support alert"

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::::::: If you forgot to apply the background correction before running your experiment, you can add the values directly to the O<sub>2</sub> analysis DL7.4 Excel template and complete your data analysis following the [[Oxygen flux - instrumental background|quality control step 2]]. »[[MiPNet24.06_Oxygen_flux_analysis_-_DatLab_7.4|Oxygen flux analysis - DatLab 7.4]]«
::::::: If you forgot to apply the background correction before running your experiment, you can add the values directly to the O<sub>2</sub> analysis DL7.4 Excel template and complete your data analysis following the [[Oxygen flux - instrumental background|quality control step 2]]. »[[MiPNet24.06_Oxygen_flux_analysis_-_DatLab_7.4|Oxygen flux analysis - DatLab 7.4]]«


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Why the oxygen levels are different between chambers prior to my experimental run?'''
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Why are the oxygen levels different between chambers prior to my experimental run?'''
::::::: After switching on the O2k, oxygen concentration values might be atypical or different between chambers if air calibration is not performed - »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]«
::::::: After switching on the O2k, oxygen concentration values might be atypical or different between chambers if air calibration is not performed - »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]«


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot support mitochondrial higher respiration flux?'''
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot support mitochondrial higher respiration flux?'''
::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«
::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«


== Removed for correction ==
== Removed for correction ==

Revision as of 08:36, 2 April 2020

While in home office mode

  • Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.


O2k-Open Support Info: improve Did you know that you can edit the background parameters in DatLab 7.4 O2 analysis Excel template?
If you forgot to apply the background correction before running your experiment, you can add the values directly to the O2 analysis DL7.4 Excel template and complete your data analysis following the quality control step 2. »Oxygen flux analysis - DatLab 7.4«
O2k-Open SupportTake care Why are the oxygen levels different between chambers prior to my experimental run?
After switching on the O2k, oxygen concentration values might be atypical or different between chambers if air calibration is not performed - » Oxygen calibration - troubleshooting«
O2k-Open SupportInfo: improve Did you know that some substrates alone cannot support mitochondrial higher respiration flux?
Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - » Pyruvate - troubleshooting«

Removed for correction

O2k-Open Support Warning: loss of data Do you perform the leaky chamber test after chamber assembly?
- An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly. - »Leaky chamber test«


» https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly


In discussion

O2k-Open Support Take care Do you know how the "switch components" approach can guide you to find what is causing your O2k not to reach the required specifications?
Do not desperate if specifications given in the O2k-Manuals are not obtained. See how to perform an experimental troubleshooting on your own.-»Switch components approach«


O2k-Open Support Warning: loss of data Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
See how to clean and dry the electrical connections of the OroboPOS-Connector.-»Cleaning the electrical connections«


O2k-Open Support alert circulars

O2k-Open Support alert circular 2020(01): in prep

New O2k-Open Support Warning: loss of data A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«


New O2k-Open SupportTake care Calibration parameters are transferred to experimental DatLab files
Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«
O2k-Open Support Warning: loss of data Do you perform O2k-Chamber volume calibration?
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«
O2k-Open Support Take care Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
The O2 solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«