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| === Preparation of 200 mM stock ===
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| TMPD (C<sub>10</sub>H<sub>16</sub>N<sub>2</sub>*2HCl): Sigma T 3134, 5 g, store at RT; FW = 237.2.
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| ::1) Weigh 47.4 mg TMPD
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| ::2) Dissolve in 1 ml H<sub>2</sub>O
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| ::3) Divide into 0.2 ml portions
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| ::4) Protect from light!
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| ::5) Store frozen at -20 °C
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| <span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial). Take care to minimize light and temperature exposure during use!</span>
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| It is important to add [[Ascorbate]] before TMPD to avoid uncontrollable autoxidation. Ascorbate maintains TMPD in a reduced state. Electrons flow from TMPD to cytochrome c.
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| For preparation and titration please visit [[Ascorbate]]
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| '''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]]
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| ::* Titration volume: 5 µl using a 25 µl syringe
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| ::* Final conc. in 2 ml O2k-chamber: 0.5 mM
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