Difference between revisions of "Tan 2011 Inflammation"
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|injuries=RONS; Oxidative Stress | |injuries=RONS; Oxidative Stress | ||
|organism=Rat | |organism=Rat | ||
|tissues=Skeletal | |tissues=Skeletal muscle | ||
|preparations=Isolated Mitochondria | |preparations=Isolated Mitochondria | ||
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Revision as of 04:25, 5 April 2012
| Tan EC, van Goor H, Bahrami S, Kozlov AV, Leixnering M, Redl H, Goris RJ (2011) Intra-arterial tert-Butyl-hydroperoxide infusion induces an exacerbated sensory response in the rat hind limb and is associated with an impaired tissue oxygen uptake. Inflammation 34: 49-57. |
Tan EC, van Goor H, Bahrami S, Kozlov AV, Leixnering M, Redl H, Goris RJ (2011) Inflammation
Abstract: The objective of this study was to investigate oxidative stress and oxygen extraction mechanisms in an animal model of continuous intra-arterial infusion of a free radical donor and in an in vitro model using isolated mitochondria. tert-Butyl-hydroperoxide (tert-BuOOH, 25 mM) was infused for 24 h in the left hind limb of rats to induce soft tissue damage (n = 8). After 7 days, we assessed local sensory response, tissue oxygen consumption, oxygen radicals, and antioxidant levels. In vitro mitochondrial function was measured after stimulation of isolated mitochondria of skeletal muscle cells with increasing doses of tert-BuOOH. tert-BuOOH infusion resulted in an increased skin temperature (P = 0.04), impaired function, and a significantly increased pain sensation (P = 0.03). Venous oxygen saturation levels (P = 0.01) and the antioxidant ceruloplasmin (P = 0.04) were increased. tert-BuOOH inhibited mitochondrial function in vitro. Induction of free radical formation in the rat hind limb results in an exacerbated sensory response and is associated with impaired oxygen extraction, which likely results from mitochondrial dysfunction caused by free radicals. • Keywords: rat model, oxygen radicals, oxygen extraction, mitochondrial dysfunction, complex regional pain syndrome
• O2k-Network Lab: AT_Vienna_Kozlov AV
Labels:
Stress:RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Rat Tissue;cell: Skeletal muscle Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
