Difference between revisions of "Template:Chemical SUIT-034 NADH mt D082"
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| | | Reoxygenation can be performed by opening the chamber, see: [[Open chamber]]. | ||
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Revision as of 18:56, 19 December 2023
| Step | Chemical(s) and link(s) | Comments |
|---|---|---|
| 1D.1 | ADP (D) | |
| 2PGM | Pyruvate (P), Glutamate (G), and Malate (M) | |
| 3D2.5 | ADP (D) | |
| 4U | SF6847 | We do not recommend the use of any other uncoupler, like Carbonyl cyanide m-chlorophenyl hydrazone, CCCP (U), due to the chemical background effect on fluorescence. |
| 5Anox | The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration. | |
| 6Myx | Myxothiazol | We do not recommend the use of any other inhibitor of complex III, like Antimycin A (Ama), due to the chemical background effect on fluorescence. |
| 7Reox | Reoxygenation can be performed by opening the chamber, see: Open chamber. |
