Difference between revisions of "Template:Chemical SUIT-034 NADH mt D082"
From Bioblast
| Line 5: | Line 5: | ||
| 1D.1 | | 1D.1 | ||
| {{Template:ADP}} | | {{Template:ADP}} | ||
| | | 40 mM stock solution. | ||
|- | |- | ||
| 2PGM | | 2PGM | ||
| Line 13: | Line 13: | ||
| 3D2.5 | | 3D2.5 | ||
| {{Template:ADP}} | | {{Template:ADP}} | ||
| | | 250 mM stock solution. | ||
|- | |- | ||
| 4U | | 4U | ||
| {{Template:SF6847}} | | {{Template:SF6847}} | ||
| We do not recommend | | We do not recommend using any other uncoupler, e.g. {{Template:CCCP}}, due to the chemical background effect on fluorescence. | ||
|- | |- | ||
| 5Anox | | 5Anox | ||
| Line 25: | Line 25: | ||
| 6Myx | | 6Myx | ||
| {{Template:Myxothiazol}} | | {{Template:Myxothiazol}} | ||
| We do not recommend | | We do not recommend using any other inhibitor of complex III, e.g. {{Template:Antimycin A}}, due to the chemical background effect on fluorescence. | ||
|- | |- | ||
| 7Reox | | 7Reox | ||
Revision as of 13:59, 21 December 2023
| Step | Chemical(s) and link(s) | Comments |
|---|---|---|
| 1D.1 | ADP (D) | 40 mM stock solution. |
| 2PGM | Pyruvate (P), Glutamate (G), and Malate (M) | |
| 3D2.5 | ADP (D) | 250 mM stock solution. |
| 4U | SF6847 | We do not recommend using any other uncoupler, e.g. Carbonyl cyanide m-chlorophenyl hydrazone, CCCP (U), due to the chemical background effect on fluorescence. |
| 5Anox | The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration. | |
| 6Myx | Myxothiazol | We do not recommend using any other inhibitor of complex III, e.g. Antimycin A (Ama), due to the chemical background effect on fluorescence. |
| 7Reox | Reoxygenation can be performed by opening the chamber, see: Open chamber. |
