Difference between revisions of "Template:Current week ecosystem agenda"

Revision as of 08:09, 20 July 2020

2020 Week 30

Jul-20 Mo

How can you compare respirometric experiments using an internal normalization?

Flux control ratios (FCR) are independent of external measurements (e.g. protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”.
- »Flux control ratio«

Read more: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«

Jul-21 Tu

Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet

from the O2k-Network »US TN Nashville Wasserman DH« and »US MN Minneapolis Hughey C«

Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print]. »Bioblast link«

Jul-22 We

Jul-23 Th

Do you know how to assess the integrity of the mitochondrial outer membrane in mitochondrial preparations?

It is possible to do so using exogenously added cytochrome c:
- »Cytochrome c control factor«

Jul-24 Fr

Jul-25 Sa

@@ Line 1: / Line 1: @@
+Week 30
-Week 29
+* '''Jul-20 Mo'''
+:{|
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+|'''How can you compare respirometric experiments using an internal normalization?'''<br>
+Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”.
+<br>
+- »[[Flux control ratio]]«
-* '''Jul-13 Mo'''
+Read more: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]«
-:{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Did you know that you can apply instrumental O<sub>2</sub> background test parameters (''i.e.'', intercept and slope) from an existing file in DatLab?''' <br>
-In the DatLab file, you can copy the Instrumental O<sub>2</sub> background test parameters directly via the ‘Copy from file’ option in the ‘Slope configuration’ window.
-For more information, see:
-- »[[Oxygen_flux_-_instrumental_background#Instrumental_O2_DLP|Copy from file]]«
 |}
 ----
-* '''Jul-14 Tu'''
+* '''Jul-21 Tu'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Alternative oxidase inhibition plays a key role in the aluminum tolerance of cultured ''Jatropha curcas'' cells'''<br>
+|'''Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet'''<br>
+:from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MN Minneapolis Hughey C]]«
+*Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print].  [[Lantier 2020 Diabetes|»Bioblast link«]]
+|}
-:from the O2k-Network »[[BR Ribeirao Preto Alberici LC]]«
-*Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on ''Jatropha curcas'' cell culture. Plant Physiol Biochem 136:92-97. [[Vicentini 2019 Plant Physiol Biochem|»Bioblast link«]]
-|}
 ----
-* '''Jul-15 We'''
+* '''Jul-22 We'''
 ----
-* '''Jul-16 Th'''
+* '''Jul-23 Th'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
-| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br>
+| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? '''
-Using different Fluorescence-Sensors and [[Filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential]], [[Calcium]], and numerous other applications open for O2k-user innovation.<br>
+It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br>
-- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Setup of the O2k-Fluo LED2-Module]]'''«
+- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«
 |}
 ----
-* '''Jul-17 Fr'''
+* '''Jul-24 Fr'''
+----
+* '''Jul-25 Sa'''